What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load. / Lear, Bianca; Marchbank, Angela; Kent, Nick; Tansey, Katherine; Andrews, Robert; Wheeler, Tara; Crompton, Rebecca; Devlin, Paul; Rogers, Hilary. J; Stead, Anthony.

In: Acta Horticulturae, 2018.

Research output: Contribution to journalMeeting abstract

Published

Standard

What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load. / Lear, Bianca; Marchbank, Angela; Kent, Nick; Tansey, Katherine; Andrews, Robert; Wheeler, Tara; Crompton, Rebecca; Devlin, Paul; Rogers, Hilary. J; Stead, Anthony.

In: Acta Horticulturae, 2018.

Research output: Contribution to journalMeeting abstract

Harvard

Lear, B, Marchbank, A, Kent, N, Tansey, K, Andrews, R, Wheeler, T, Crompton, R, Devlin, P, Rogers, HJ & Stead, A 2018, 'What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load', Acta Horticulturae.

APA

Vancouver

Lear B, Marchbank A, Kent N, Tansey K, Andrews R, Wheeler T et al. What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load. Acta Horticulturae. 2018.

Author

Lear, Bianca ; Marchbank, Angela ; Kent, Nick ; Tansey, Katherine ; Andrews, Robert ; Wheeler, Tara ; Crompton, Rebecca ; Devlin, Paul ; Rogers, Hilary. J ; Stead, Anthony. / What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load. In: Acta Horticulturae. 2018.

BibTeX

@article{1196a07cd2084e59925122c8dcc16ecc,
title = "What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load",
abstract = "Bent-neck or ‘necking’ is a phenomenon often seen in cut roses, whereby the flower head droops due to a bending of the peduncle. Necking is thought to be caused by a blockage of the xylem, either due to an air embolism or an accumulation of microbial populations, limiting water uptake to the flower head and causing the stem to bend. Despite the use of biocides to reduce microbial load, necking still remains an issue for the cut flower sector and roses continue to die prematurely. As the occurrence of necking varies widely within and between cultivars, a transcriptome analysis of cut Rosa hybrida cultivar ‘H30’ has been carried out to investigate the potential responses in the rose tissue and the mechanisms involved. Transcriptomes from peduncle samples at three stages of necking (straight,90°) were sequenced using next generation sequencing to produce over 100 million reads per stage and generating nearly 300, 000 contigs following de novo assembly. Differential expression analysis of the three different stages of necking identified more than 900 genes that changed in transcription during necking (>90°) compared to straight stems. Functional analysis of these differentially expressed genes has revealed multiple pathways that may contribute to the necking response. The variation in microbial loads associated with stem ends has also been analysed and together with the transcriptomics data may help gain a better understanding of the process of necking.",
author = "Bianca Lear and Angela Marchbank and Nick Kent and Katherine Tansey and Robert Andrews and Tara Wheeler and Rebecca Crompton and Paul Devlin and Rogers, {Hilary. J} and Anthony Stead",
year = "2018",
language = "English",
journal = "Acta Horticulturae",
issn = "0567-7572",
publisher = "International Society for Horticultural Science",

}

RIS

TY - JOUR

T1 - What causes peduncle necking in cut Rosa hybrida? Transcriptome analysis of rose stem tissue and analysis of microbial load

AU - Lear, Bianca

AU - Marchbank, Angela

AU - Kent, Nick

AU - Tansey, Katherine

AU - Andrews, Robert

AU - Wheeler, Tara

AU - Crompton, Rebecca

AU - Devlin, Paul

AU - Rogers, Hilary. J

AU - Stead, Anthony

PY - 2018

Y1 - 2018

N2 - Bent-neck or ‘necking’ is a phenomenon often seen in cut roses, whereby the flower head droops due to a bending of the peduncle. Necking is thought to be caused by a blockage of the xylem, either due to an air embolism or an accumulation of microbial populations, limiting water uptake to the flower head and causing the stem to bend. Despite the use of biocides to reduce microbial load, necking still remains an issue for the cut flower sector and roses continue to die prematurely. As the occurrence of necking varies widely within and between cultivars, a transcriptome analysis of cut Rosa hybrida cultivar ‘H30’ has been carried out to investigate the potential responses in the rose tissue and the mechanisms involved. Transcriptomes from peduncle samples at three stages of necking (straight,90°) were sequenced using next generation sequencing to produce over 100 million reads per stage and generating nearly 300, 000 contigs following de novo assembly. Differential expression analysis of the three different stages of necking identified more than 900 genes that changed in transcription during necking (>90°) compared to straight stems. Functional analysis of these differentially expressed genes has revealed multiple pathways that may contribute to the necking response. The variation in microbial loads associated with stem ends has also been analysed and together with the transcriptomics data may help gain a better understanding of the process of necking.

AB - Bent-neck or ‘necking’ is a phenomenon often seen in cut roses, whereby the flower head droops due to a bending of the peduncle. Necking is thought to be caused by a blockage of the xylem, either due to an air embolism or an accumulation of microbial populations, limiting water uptake to the flower head and causing the stem to bend. Despite the use of biocides to reduce microbial load, necking still remains an issue for the cut flower sector and roses continue to die prematurely. As the occurrence of necking varies widely within and between cultivars, a transcriptome analysis of cut Rosa hybrida cultivar ‘H30’ has been carried out to investigate the potential responses in the rose tissue and the mechanisms involved. Transcriptomes from peduncle samples at three stages of necking (straight,90°) were sequenced using next generation sequencing to produce over 100 million reads per stage and generating nearly 300, 000 contigs following de novo assembly. Differential expression analysis of the three different stages of necking identified more than 900 genes that changed in transcription during necking (>90°) compared to straight stems. Functional analysis of these differentially expressed genes has revealed multiple pathways that may contribute to the necking response. The variation in microbial loads associated with stem ends has also been analysed and together with the transcriptomics data may help gain a better understanding of the process of necking.

M3 - Meeting abstract

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

ER -