The regulation of carotenoid formation in tomato fruit. / Enfissi, Eugenia; Nogueira, Marilise; Bramley, Peter; Fraser, Paul.

In: Plant Journal, Vol. 89, No. 4, 02.2017, p. 774–788.

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Abstract

Carotenoid biosynthesis in plants includes a complex series of desaturation/isomerisation reactions, catalysed by four independent enzymes. In bacteria and fungi one desaturase/isomerase enzyme completes the same series of reactions. In the present study, a bacterial desaturase (crtI) from Pantoea ananatis has been overexpressed in the tangerine mutant of tomato (Solanum lycopersicon) which accumulates cis carotene isomers in the fruit due to a defective isomerase (CRTISO) and the old gold crimson (ogc) tomato mutant, which is defective in the fruit-enhanced lycopene β-cyclase (CYCB). Comprehensive molecular and biochemical characterisation of the resulting lines expressing crtI has revealed negative feedback mechanisms, acting predominantly at the level of phytoene synthase-1 (PSY1), and feed-forward mechanisms inducing cyclisation. In both cases, altered transcription appears to be the progenitor, with subsequent post-transcriptional modulation highlighting the complexity of the processes involved in modulating carotenoid homeostasis in plant tissues.
Original languageEnglish
Pages (from-to)774–788
Number of pages15
JournalPlant Journal
Volume89
Issue number4
Early online date19 Nov 2016
DOIs
Publication statusPublished - Feb 2017
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

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