Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival. / Loudya, Naresh; Okunola, Tolulope; He, Jia; jarvis, paul; Lopez Juez, Enrique.

In: Philosophical Transactions of the Royal Society of London B: Biological Sciences, Vol. 375, No. 1801, RSTB-2019-0400.R1, 22.06.2020, p. 1-16.

Research output: Contribution to journalArticlepeer-review

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Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival. / Loudya, Naresh; Okunola, Tolulope; He, Jia; jarvis, paul; Lopez Juez, Enrique.

In: Philosophical Transactions of the Royal Society of London B: Biological Sciences, Vol. 375, No. 1801, RSTB-2019-0400.R1, 22.06.2020, p. 1-16.

Research output: Contribution to journalArticlepeer-review

Harvard

Loudya, N, Okunola, T, He, J, jarvis, P & Lopez Juez, E 2020, 'Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival', Philosophical Transactions of the Royal Society of London B: Biological Sciences, vol. 375, no. 1801, RSTB-2019-0400.R1, pp. 1-16. https://doi.org/10.1098/rstb.2019.0400

APA

Loudya, N., Okunola, T., He, J., jarvis, P., & Lopez Juez, E. (2020). Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival. Philosophical Transactions of the Royal Society of London B: Biological Sciences, 375(1801), 1-16. [RSTB-2019-0400.R1]. https://doi.org/10.1098/rstb.2019.0400

Vancouver

Loudya N, Okunola T, He J, jarvis P, Lopez Juez E. Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival. Philosophical Transactions of the Royal Society of London B: Biological Sciences. 2020 Jun 22;375(1801):1-16. RSTB-2019-0400.R1. https://doi.org/10.1098/rstb.2019.0400

Author

Loudya, Naresh ; Okunola, Tolulope ; He, Jia ; jarvis, paul ; Lopez Juez, Enrique. / Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival. In: Philosophical Transactions of the Royal Society of London B: Biological Sciences. 2020 ; Vol. 375, No. 1801. pp. 1-16.

BibTeX

@article{9bc597b752624847a9b6be8fe02a8964,
title = "Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival",
abstract = "Defects in chloroplast development are “retrograde-signalled{"} to the nucleus, reducing synthesis of photosynthetic or related proteins. The Arabidopsis cue8 mutant manifests virescence, a slow-greening phenotype, and is defective at an early stage in plastid development. Greening cotyledons or early leaf cells of cue8 exhibit immature chloroplasts which fail to fill the available cellular space. Such chloroplasts show reduced expression of genes of photosynthetic function, dependent on the plastid-encoded RNA polymerase (PEP), while expression of genes of housekeeping function driven by the nucleus-encoded polymerase (NEP) is elevated, a phenotype shared with mutants in plastid genetic functions. We attribute this phenotype to reduced expression of specific PEP-controlling sigma factors, elevated expression of RPOT (NEP) genes, and maintained replication of plastid genomes (resulting in densely coalesced nucleoids in the mutant), i.e. it is due to an anterograde nucleus-to-chloroplast correction, analogous to retention of a juvenile plastid state. Mutants in plastid protein import components, particularly those involved in housekeeping protein import, also show this {"}retro-anterograde{"} correction. Loss of CUE8 also causes changes in mRNA editing. The overall response has strong fitness value: loss of GUN1, an integrator of retrograde signalling, abolishes elements of it (albeit not others, including editing changes), causing bleaching and eventual seedling lethality upon cue8 gun1. This highlights the adaptive significance of virescence and retrograde signalling.",
author = "Naresh Loudya and Tolulope Okunola and Jia He and paul jarvis and {Lopez Juez}, Enrique",
year = "2020",
month = jun,
day = "22",
doi = "10.1098/rstb.2019.0400",
language = "English",
volume = "375",
pages = "1--16",
journal = "Philosophical Transactions of the Royal Society of London B: Biological Sciences",
issn = "0962-8436",
publisher = "Royal Society of London",
number = "1801",

}

RIS

TY - JOUR

T1 - Retrograde signalling in a virescent mutant triggers an anterograde delay of chloroplast biogenesis that requires GUN1 and is essential for survival

AU - Loudya, Naresh

AU - Okunola, Tolulope

AU - He, Jia

AU - jarvis, paul

AU - Lopez Juez, Enrique

PY - 2020/6/22

Y1 - 2020/6/22

N2 - Defects in chloroplast development are “retrograde-signalled" to the nucleus, reducing synthesis of photosynthetic or related proteins. The Arabidopsis cue8 mutant manifests virescence, a slow-greening phenotype, and is defective at an early stage in plastid development. Greening cotyledons or early leaf cells of cue8 exhibit immature chloroplasts which fail to fill the available cellular space. Such chloroplasts show reduced expression of genes of photosynthetic function, dependent on the plastid-encoded RNA polymerase (PEP), while expression of genes of housekeeping function driven by the nucleus-encoded polymerase (NEP) is elevated, a phenotype shared with mutants in plastid genetic functions. We attribute this phenotype to reduced expression of specific PEP-controlling sigma factors, elevated expression of RPOT (NEP) genes, and maintained replication of plastid genomes (resulting in densely coalesced nucleoids in the mutant), i.e. it is due to an anterograde nucleus-to-chloroplast correction, analogous to retention of a juvenile plastid state. Mutants in plastid protein import components, particularly those involved in housekeeping protein import, also show this "retro-anterograde" correction. Loss of CUE8 also causes changes in mRNA editing. The overall response has strong fitness value: loss of GUN1, an integrator of retrograde signalling, abolishes elements of it (albeit not others, including editing changes), causing bleaching and eventual seedling lethality upon cue8 gun1. This highlights the adaptive significance of virescence and retrograde signalling.

AB - Defects in chloroplast development are “retrograde-signalled" to the nucleus, reducing synthesis of photosynthetic or related proteins. The Arabidopsis cue8 mutant manifests virescence, a slow-greening phenotype, and is defective at an early stage in plastid development. Greening cotyledons or early leaf cells of cue8 exhibit immature chloroplasts which fail to fill the available cellular space. Such chloroplasts show reduced expression of genes of photosynthetic function, dependent on the plastid-encoded RNA polymerase (PEP), while expression of genes of housekeeping function driven by the nucleus-encoded polymerase (NEP) is elevated, a phenotype shared with mutants in plastid genetic functions. We attribute this phenotype to reduced expression of specific PEP-controlling sigma factors, elevated expression of RPOT (NEP) genes, and maintained replication of plastid genomes (resulting in densely coalesced nucleoids in the mutant), i.e. it is due to an anterograde nucleus-to-chloroplast correction, analogous to retention of a juvenile plastid state. Mutants in plastid protein import components, particularly those involved in housekeeping protein import, also show this "retro-anterograde" correction. Loss of CUE8 also causes changes in mRNA editing. The overall response has strong fitness value: loss of GUN1, an integrator of retrograde signalling, abolishes elements of it (albeit not others, including editing changes), causing bleaching and eventual seedling lethality upon cue8 gun1. This highlights the adaptive significance of virescence and retrograde signalling.

U2 - 10.1098/rstb.2019.0400

DO - 10.1098/rstb.2019.0400

M3 - Article

VL - 375

SP - 1

EP - 16

JO - Philosophical Transactions of the Royal Society of London B: Biological Sciences

JF - Philosophical Transactions of the Royal Society of London B: Biological Sciences

SN - 0962-8436

IS - 1801

M1 - RSTB-2019-0400.R1

ER -