Inhibition of Myostatin Reduces Collagen Deposition in a Mouse Model of Oculopharyngeal Muscular Dystrophy (OPMD) With Established Disease. / Harish, Pradeep; Forrest, Leysa; Herath, Shanthi; Dickson, George; Malerba, Alberto; Popplewell, Linda.

In: Frontiers in Physiology, Vol. 11, 184, 05.03.2020, p. 1-8.

Research output: Contribution to journalArticle

Published

Abstract

Background: Oculopharyngeal muscular dystrophy (OPMD) is a late-onset muscle
disease presented by ptosis, dysphagia, and limb weakness. Affected muscles display increased fibrosis and atrophy, with characteristic inclusion bodies in the nucleus.Myostatin is a negative regulator of muscle mass, and inhibition of myostatin has been demonstrated to improve symptoms in models of muscular dystrophy.
Methods: We systemically administered a monoclonal antibody to block myostatin in the A17 mouse model of OPMD at 42 weeks of age. The mice were administered a weekly dose of 10 mg/kg RK35 intraperitonially for 10 weeks, following which serum and histological analyses were performed on muscle samples.
Results: The administration of the antibody resulted in a significant decrease in serum myostatin and collagen deposition in muscles. However, minimal effects on body mass, muscle mass and myofiber diameter, or the density of intranuclear inclusions (INIs) (a hallmark of disease progression of OPMD) were observed.
Conclusion: This study demonstrates that inhibition of myostatin does not revert
muscle atrophy in a mouse model with established OPMD disease, but is effective at reducing observed histological markers of fibrosis in the treated muscles.
Original languageEnglish
Article number184
Pages (from-to)1-8
Number of pages8
JournalFrontiers in Physiology
Volume11
DOIs
Publication statusPublished - 5 Mar 2020
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

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