Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. / Nogueira, Marilise; Enfissi, Eugenia; martinez, Maria; Driller, Lothar; Schuch, Wolfgang; Sandmann, Gerhard; Fraser, Paul.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 41, 10.10.2017, p. 10876–10881.

Research output: Contribution to journalArticle

Published

Documents

Abstract

Ketocarotenoids are high-value pigments used commercially across multiple industrial sectors as colorants and supplements. Chemical synthesis using petrochemical-derived precursors remains the production method of choice. Aquaculture is an example where ketocarotenoid supplementation of feed is necessary to achieve product viability. The biosynthesis of ketocarotenoids, such as canthaxanthin, phoenicoxanthin, or astaxanthin in plants is rare. In the present study, complex engineering of the carotenoid pathway has been performed to produce high-value ketocarotenoids in tomato fruit (3.0 mg/g dry weight). The strategy adopted involved pathway extension beyond β-carotene through the expression of the β-carotene hydroxylase (CrtZ) and oxyxgenase (CrtW) from Brevundimonas sp. in tomato fruit, followed by β-carotene enhancement through the introgression of a lycopene β-cyclase (β-Cyc) allele from a Solanum galapagense background. Detailed biochemical analysis, carried out using chromatographic, UV/VIS, and MS approaches, identified the predominant carotenoid as fatty acid (C14:0 and C16:0) esters of phoenicoxanthin, present in the S stereoisomer configuration. Under a field-like environment with low resource input, scalability was shown with the potential to deliver 23 kg of ketocarotenoid/hectare. To illustrate the potential of this “generally recognized as safe” material with minimal, low-energy bioprocessing, two independent aquaculture trials were performed. The plant-based feeds developed were more efficient than the synthetic feed to color trout flesh (up to twofold increase in the retention of the main ketocarotenoids in the fish fillets). This achievement has the potential to create a new paradigm in the renewable production of economically competitive feed additives for the aquaculture industry and beyond.
Original languageEnglish
Pages (from-to)10876–10881
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume114
Issue number41
Early online date25 Sep 2017
DOIs
Publication statusPublished - 10 Oct 2017
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

ID: 28691693