Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis

Zhensheng Pan; Richard Barry; Alexey Lipkin; Mikhail Soloviev

doi:10.1186/1471-2199-8-32

Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis

Zhensheng Pan, Richard Barry, Alexey Lipkin, Mikhail Soloviev

Research output: Contribution to journal › Article › peer-review

Original language	English
Pages (from-to)	32
Journal	BMC Molecular Biology
Volume	8
DOIs	https://doi.org/10.1186/1471-2199-8-32
Publication status	Published - 2007

Keywords

Amino Acid Sequence
Animals
Base Sequence
Cloning, Molecular
Consensus Sequence
DNA Primers
DNA, Complementary
Databases, Protein
Phylogeny
Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
Spiders
Toxins, Biological
Transition Temperature

Access to Document

10.1186/1471-2199-8-32

Cite this

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title = "Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis",

keywords = "Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, Consensus Sequence, DNA Primers, DNA, Complementary, Databases, Protein, Phylogeny, Polymerase Chain Reaction, Sequence Alignment, Sequence Analysis, DNA, Spiders, Toxins, Biological, Transition Temperature",

author = "Zhensheng Pan and Richard Barry and Alexey Lipkin and Mikhail Soloviev",

year = "2007",

doi = "10.1186/1471-2199-8-32",

language = "English",

volume = "8",

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journal = "BMC Molecular Biology",

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T1 - Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis

AU - Pan, Zhensheng

AU - Barry, Richard

AU - Lipkin, Alexey

AU - Soloviev, Mikhail

PY - 2007

Y1 - 2007

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - Cloning, Molecular

KW - Consensus Sequence

KW - DNA Primers

KW - DNA, Complementary

KW - Databases, Protein

KW - Phylogeny

KW - Polymerase Chain Reaction

KW - Sequence Alignment

KW - Sequence Analysis, DNA

KW - Spiders

KW - Toxins, Biological

KW - Transition Temperature

U2 - 10.1186/1471-2199-8-32

DO - 10.1186/1471-2199-8-32

M3 - Article

C2 - 17498297

SN - 1471-2199

VL - 8

SP - 32

JO - BMC Molecular Biology

JF - BMC Molecular Biology

ER -