Virulence and intermediate resistance to high‐end antibiotic (teicoplanin) among coagulase‐negative staphylococci sourced from retail market fish. / Muneeb, K.H. ; Sudha, S.; Sivaraman, G.K. ; Shome, Bibek; Cole, Jennifer; Holmes, Mark.

In: Archives of microbiology, Vol. 2021, 01.09.2021.

Research output: Contribution to journalArticlepeer-review

Published

Standard

Virulence and intermediate resistance to high‐end antibiotic (teicoplanin) among coagulase‐negative staphylococci sourced from retail market fish. / Muneeb, K.H. ; Sudha, S.; Sivaraman, G.K. ; Shome, Bibek; Cole, Jennifer; Holmes, Mark.

In: Archives of microbiology, Vol. 2021, 01.09.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

Muneeb, K.H. ; Sudha, S. ; Sivaraman, G.K. ; Shome, Bibek ; Cole, Jennifer ; Holmes, Mark. / Virulence and intermediate resistance to high‐end antibiotic (teicoplanin) among coagulase‐negative staphylococci sourced from retail market fish. In: Archives of microbiology. 2021 ; Vol. 2021.

BibTeX

@article{41af211faf13452f855dab04ccbf14d3,
title = "Virulence and intermediate resistance to high‐end antibiotic (teicoplanin) among coagulase‐negative staphylococci sourced from retail market fish",
abstract = "This study reports the distribution of enterotoxigenic determinants among staphylococci and the susceptibility of staphy- lococci to various classes of antibiotics. We observed all the isolates as resistant to beta-lactam antibiotics and a few as resistant to non-beta-lactam antibiotics such as clindamycin (47.4%), erythromycin (44.7%), gentamicin (23.7%), norfloxacin (34.2%), tetracycline (26.3%), trimethoprim-sulfamethoxazole (15.8%) etc. The resistance of S. sciuri (n = 1) and S. haemo- lyticus (n = 1) to rifampicin and intermediate resistance of S. gallinarum (n = 2) to teicoplanin, a high-end antibiotic, are also observed in this study. The multidrug-resistance (≥ 3 classes of antibiotics) was recorded in 23 (60.5%) isolates. The virulomes such as sea, seb, seg and sei were identified predominantly in S. haemolyticus. Surprisingly, certain isolates which were phenotypically confirmed as biofilm-producers by Congo red agar (CRA) test did not harbor biofilm-associated loci. This implies the protein-mediated mechanism of biofilm formation as an alternative to polysaccharide intercellular adhesin (PIA) in staphylococci. However, icaAD locus which encodes PIA was identified in 10 (26.3%) isolates and the eno locus, encoding elastin-binding protein which can accelerate the biofilm production, is identified in all the isolates. The posses- sion of type V SCCmec elements by the S. haemolyticus (15.8%) raised the concern about the rapid dissemination of mecA gene to other species of staphylococci including the virulent S. aureus. In short, this study acknowledges the toxigenicity of coagulase-negative staphylococci (CoNS). Through this study, surveillance of antimicrobial resistance and transference of virulomes in staphylococci is warranted.",
keywords = "Coagulase-negative staphylococci, Virulence genes, mecA gene, Type V SCCmec elements, Enterotoxins",
author = "K.H. Muneeb and S. Sudha and G.K. Sivaraman and Bibek Shome and Jennifer Cole and Mark Holmes",
year = "2021",
month = sep,
day = "1",
doi = "10.1007/s00203-021-02558-2",
language = "English",
volume = "2021",
journal = "Archives of microbiology",
issn = "0302-8933",
publisher = "Springer Verlag",

}

RIS

TY - JOUR

T1 - Virulence and intermediate resistance to high‐end antibiotic (teicoplanin) among coagulase‐negative staphylococci sourced from retail market fish

AU - Muneeb, K.H.

AU - Sudha, S.

AU - Sivaraman, G.K.

AU - Shome, Bibek

AU - Cole, Jennifer

AU - Holmes, Mark

PY - 2021/9/1

Y1 - 2021/9/1

N2 - This study reports the distribution of enterotoxigenic determinants among staphylococci and the susceptibility of staphy- lococci to various classes of antibiotics. We observed all the isolates as resistant to beta-lactam antibiotics and a few as resistant to non-beta-lactam antibiotics such as clindamycin (47.4%), erythromycin (44.7%), gentamicin (23.7%), norfloxacin (34.2%), tetracycline (26.3%), trimethoprim-sulfamethoxazole (15.8%) etc. The resistance of S. sciuri (n = 1) and S. haemo- lyticus (n = 1) to rifampicin and intermediate resistance of S. gallinarum (n = 2) to teicoplanin, a high-end antibiotic, are also observed in this study. The multidrug-resistance (≥ 3 classes of antibiotics) was recorded in 23 (60.5%) isolates. The virulomes such as sea, seb, seg and sei were identified predominantly in S. haemolyticus. Surprisingly, certain isolates which were phenotypically confirmed as biofilm-producers by Congo red agar (CRA) test did not harbor biofilm-associated loci. This implies the protein-mediated mechanism of biofilm formation as an alternative to polysaccharide intercellular adhesin (PIA) in staphylococci. However, icaAD locus which encodes PIA was identified in 10 (26.3%) isolates and the eno locus, encoding elastin-binding protein which can accelerate the biofilm production, is identified in all the isolates. The posses- sion of type V SCCmec elements by the S. haemolyticus (15.8%) raised the concern about the rapid dissemination of mecA gene to other species of staphylococci including the virulent S. aureus. In short, this study acknowledges the toxigenicity of coagulase-negative staphylococci (CoNS). Through this study, surveillance of antimicrobial resistance and transference of virulomes in staphylococci is warranted.

AB - This study reports the distribution of enterotoxigenic determinants among staphylococci and the susceptibility of staphy- lococci to various classes of antibiotics. We observed all the isolates as resistant to beta-lactam antibiotics and a few as resistant to non-beta-lactam antibiotics such as clindamycin (47.4%), erythromycin (44.7%), gentamicin (23.7%), norfloxacin (34.2%), tetracycline (26.3%), trimethoprim-sulfamethoxazole (15.8%) etc. The resistance of S. sciuri (n = 1) and S. haemo- lyticus (n = 1) to rifampicin and intermediate resistance of S. gallinarum (n = 2) to teicoplanin, a high-end antibiotic, are also observed in this study. The multidrug-resistance (≥ 3 classes of antibiotics) was recorded in 23 (60.5%) isolates. The virulomes such as sea, seb, seg and sei were identified predominantly in S. haemolyticus. Surprisingly, certain isolates which were phenotypically confirmed as biofilm-producers by Congo red agar (CRA) test did not harbor biofilm-associated loci. This implies the protein-mediated mechanism of biofilm formation as an alternative to polysaccharide intercellular adhesin (PIA) in staphylococci. However, icaAD locus which encodes PIA was identified in 10 (26.3%) isolates and the eno locus, encoding elastin-binding protein which can accelerate the biofilm production, is identified in all the isolates. The posses- sion of type V SCCmec elements by the S. haemolyticus (15.8%) raised the concern about the rapid dissemination of mecA gene to other species of staphylococci including the virulent S. aureus. In short, this study acknowledges the toxigenicity of coagulase-negative staphylococci (CoNS). Through this study, surveillance of antimicrobial resistance and transference of virulomes in staphylococci is warranted.

KW - Coagulase-negative staphylococci

KW - Virulence genes

KW - mecA gene

KW - Type V SCCmec elements

KW - Enterotoxins

U2 - 10.1007/s00203-021-02558-2

DO - 10.1007/s00203-021-02558-2

M3 - Article

VL - 2021

JO - Archives of microbiology

JF - Archives of microbiology

SN - 0302-8933

ER -