Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo. / Pollard, Andrea; Pitsillides, Andrew ; Portugal, Steve.

In: Physiological and Biochemical Zoology, Vol. 89, No. 4, 16.05.2016, p. 331-339.

Research output: Contribution to journalArticle

E-pub ahead of print

Standard

Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo. / Pollard, Andrea; Pitsillides, Andrew ; Portugal, Steve.

In: Physiological and Biochemical Zoology, Vol. 89, No. 4, 16.05.2016, p. 331-339.

Research output: Contribution to journalArticle

Harvard

Pollard, A, Pitsillides, A & Portugal, S 2016, 'Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo', Physiological and Biochemical Zoology, vol. 89, no. 4, pp. 331-339. https://doi.org/10.1086/687228

APA

Pollard, A., Pitsillides, A., & Portugal, S. (2016). Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo. Physiological and Biochemical Zoology, 89(4), 331-339. https://doi.org/10.1086/687228

Vancouver

Pollard A, Pitsillides A, Portugal S. Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo. Physiological and Biochemical Zoology. 2016 May 16;89(4):331-339. https://doi.org/10.1086/687228

Author

Pollard, Andrea ; Pitsillides, Andrew ; Portugal, Steve. / Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo. In: Physiological and Biochemical Zoology. 2016 ; Vol. 89, No. 4. pp. 331-339.

BibTeX

@article{e973b78d4378432fb6dd10818920ae2d,
title = "Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo",
abstract = "Avian embryos are a commonly used model system for developmental studies, but monitoring of physiological parameters such as heart rate (HR) and movement in ovo poses a challenge to researchers. These are also increasingly common research objectives for ecological and embryo behavior studies in oviparous species. We therefore explored the validity of a new digital egg-monitoring system for the noninvasive monitoring of these parameters. We tested the relationship between frequency-of-movement values gathered by digital monitoring and those gathered by the current standard method, which is comparatively invasive and requires egg windowing, and demonstrated that the digital monitoring method effectively distinguishes individual movements but cannot reliably monitor HR in actively motile embryos. We therefore provide recommendations for the appropriate use of this technique for avian physiologists. We also applied the digital monitoring method to reveal how frequency of movement varies throughout prenatal ontogeny in the chicken and showed that commonly used protocols in developmental studies can themselves alter motility; egg windowing and application of light modulate frequency of movement. Recent work has revealed the importance of embryo motility in regulating gene expression and cellular activity during developmental processes. Together with our data, this highlights the value of noninvasive monitoring methods and the importance of controlling for altered embryo motility/behavior in developmental studies.",
author = "Andrea Pollard and Andrew Pitsillides and Steve Portugal",
year = "2016",
month = "5",
day = "16",
doi = "10.1086/687228",
language = "English",
volume = "89",
pages = "331--339",
journal = "Physiological and Biochemical Zoology",
issn = "1522-2152",
publisher = "University of Chicago",
number = "4",

}

RIS

TY - JOUR

T1 - Validating a Noninvasive Technique for Monitoring Embryo Movement In Ovo

AU - Pollard, Andrea

AU - Pitsillides, Andrew

AU - Portugal, Steve

PY - 2016/5/16

Y1 - 2016/5/16

N2 - Avian embryos are a commonly used model system for developmental studies, but monitoring of physiological parameters such as heart rate (HR) and movement in ovo poses a challenge to researchers. These are also increasingly common research objectives for ecological and embryo behavior studies in oviparous species. We therefore explored the validity of a new digital egg-monitoring system for the noninvasive monitoring of these parameters. We tested the relationship between frequency-of-movement values gathered by digital monitoring and those gathered by the current standard method, which is comparatively invasive and requires egg windowing, and demonstrated that the digital monitoring method effectively distinguishes individual movements but cannot reliably monitor HR in actively motile embryos. We therefore provide recommendations for the appropriate use of this technique for avian physiologists. We also applied the digital monitoring method to reveal how frequency of movement varies throughout prenatal ontogeny in the chicken and showed that commonly used protocols in developmental studies can themselves alter motility; egg windowing and application of light modulate frequency of movement. Recent work has revealed the importance of embryo motility in regulating gene expression and cellular activity during developmental processes. Together with our data, this highlights the value of noninvasive monitoring methods and the importance of controlling for altered embryo motility/behavior in developmental studies.

AB - Avian embryos are a commonly used model system for developmental studies, but monitoring of physiological parameters such as heart rate (HR) and movement in ovo poses a challenge to researchers. These are also increasingly common research objectives for ecological and embryo behavior studies in oviparous species. We therefore explored the validity of a new digital egg-monitoring system for the noninvasive monitoring of these parameters. We tested the relationship between frequency-of-movement values gathered by digital monitoring and those gathered by the current standard method, which is comparatively invasive and requires egg windowing, and demonstrated that the digital monitoring method effectively distinguishes individual movements but cannot reliably monitor HR in actively motile embryos. We therefore provide recommendations for the appropriate use of this technique for avian physiologists. We also applied the digital monitoring method to reveal how frequency of movement varies throughout prenatal ontogeny in the chicken and showed that commonly used protocols in developmental studies can themselves alter motility; egg windowing and application of light modulate frequency of movement. Recent work has revealed the importance of embryo motility in regulating gene expression and cellular activity during developmental processes. Together with our data, this highlights the value of noninvasive monitoring methods and the importance of controlling for altered embryo motility/behavior in developmental studies.

U2 - 10.1086/687228

DO - 10.1086/687228

M3 - Article

VL - 89

SP - 331

EP - 339

JO - Physiological and Biochemical Zoology

JF - Physiological and Biochemical Zoology

SN - 1522-2152

IS - 4

ER -