γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis. / Kállai, Brigitta M; Kourová, Hana; Chumová, Jana; Papdi, Csaba; Trögelová, Lucie; Kofroňová, Olga; Hozák, Pavel; Filimonenko, Vlada; Mészáros , Tamas; Magyar , Zoltan; Bogre, Laszlo; Binarová, Pavla.

In: Journal of Experimental Botany, 06.11.2019.

Research output: Contribution to journalArticle

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γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis. / Kállai, Brigitta M; Kourová, Hana; Chumová, Jana; Papdi, Csaba; Trögelová, Lucie; Kofroňová, Olga; Hozák, Pavel; Filimonenko, Vlada; Mészáros , Tamas; Magyar , Zoltan; Bogre, Laszlo; Binarová, Pavla.

In: Journal of Experimental Botany, 06.11.2019.

Research output: Contribution to journalArticle

Harvard

Kállai, BM, Kourová, H, Chumová, J, Papdi, C, Trögelová, L, Kofroňová, O, Hozák, P, Filimonenko, V, Mészáros , T, Magyar , Z, Bogre, L & Binarová, P 2019, 'γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis', Journal of Experimental Botany. https://doi.org/10.1093/jxb/erz498

APA

Kállai, B. M., Kourová, H., Chumová, J., Papdi, C., Trögelová, L., Kofroňová, O., ... Binarová, P. (2019). γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis. Journal of Experimental Botany, [erz498]. https://doi.org/10.1093/jxb/erz498

Vancouver

Kállai BM, Kourová H, Chumová J, Papdi C, Trögelová L, Kofroňová O et al. γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis. Journal of Experimental Botany. 2019 Nov 6. erz498. https://doi.org/10.1093/jxb/erz498

Author

Kállai, Brigitta M ; Kourová, Hana ; Chumová, Jana ; Papdi, Csaba ; Trögelová, Lucie ; Kofroňová, Olga ; Hozák, Pavel ; Filimonenko, Vlada ; Mészáros , Tamas ; Magyar , Zoltan ; Bogre, Laszlo ; Binarová, Pavla. / γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis. In: Journal of Experimental Botany. 2019.

BibTeX

@article{7e0764274eff4878afbc474233944d1e,
title = "γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis",
abstract = "γ-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it functions also in nuclear processes and in cell division control that are independent of its canonical role. We found that in Arabidopsis thaliana γ-tubulin interacts specifically with E2FA, E2FB, and E2FC transcription factors both in vitro and in vivo. The interaction of γ-tubulin with the E2Fs is not reduced in the presence of the dimerization partners (DPs) and, in agreement, we found that γ-tubulin interaction with E2Fs does not require the dimerization domain. γ-Tubulin associates with the promoters of E2F-regulated cell cycle genes in an E2F dependent manner, likely in complex with the E2F/DP heterodimer. The upregulation of E2F targets; PCNA, ORC2, CDKB1;1 and CCS52A under γ-tubulin silencing suggests a repressive function for γ-tubulin at G1/S, G2/M and endocycle, which is consistent with an excess of cell division in some cells and enhanced endoreduplication in others in the shoot and young leaves of γ-tubulin RNAi plants. Altogether, our data show ternary interaction of γ-tubulin with E2F/DP heterodimer and suggest a repressive role for γ-tubulin with E2Fs in controlling mitotic activity and endoreduplication during plant development.",
keywords = "Arabidopsis, E2F transcription factors, endoreduplication, gene expression, proliferation, γ-Tubulin",
author = "K{\'a}llai, {Brigitta M} and Hana Kourov{\'a} and Jana Chumov{\'a} and Csaba Papdi and Lucie Tr{\"o}gelov{\'a} and Olga Kofroňov{\'a} and Pavel Hoz{\'a}k and Vlada Filimonenko and Tamas M{\'e}sz{\'a}ros and Zoltan Magyar and Laszlo Bogre and Pavla Binarov{\'a}",
year = "2019",
month = "11",
day = "6",
doi = "10.1093/jxb/erz498",
language = "English",
journal = "Journal of Experimental Botany",
issn = "0022-0957",
publisher = "OXFORD UNIV PRESS",

}

RIS

TY - JOUR

T1 - γ-Tubulin interacts with E2FA, E2FB and E2FC transcription factors, regulates proliferation and endocycle in Arabidopsis

AU - Kállai, Brigitta M

AU - Kourová, Hana

AU - Chumová, Jana

AU - Papdi, Csaba

AU - Trögelová, Lucie

AU - Kofroňová, Olga

AU - Hozák, Pavel

AU - Filimonenko, Vlada

AU - Mészáros , Tamas

AU - Magyar , Zoltan

AU - Bogre, Laszlo

AU - Binarová, Pavla

PY - 2019/11/6

Y1 - 2019/11/6

N2 - γ-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it functions also in nuclear processes and in cell division control that are independent of its canonical role. We found that in Arabidopsis thaliana γ-tubulin interacts specifically with E2FA, E2FB, and E2FC transcription factors both in vitro and in vivo. The interaction of γ-tubulin with the E2Fs is not reduced in the presence of the dimerization partners (DPs) and, in agreement, we found that γ-tubulin interaction with E2Fs does not require the dimerization domain. γ-Tubulin associates with the promoters of E2F-regulated cell cycle genes in an E2F dependent manner, likely in complex with the E2F/DP heterodimer. The upregulation of E2F targets; PCNA, ORC2, CDKB1;1 and CCS52A under γ-tubulin silencing suggests a repressive function for γ-tubulin at G1/S, G2/M and endocycle, which is consistent with an excess of cell division in some cells and enhanced endoreduplication in others in the shoot and young leaves of γ-tubulin RNAi plants. Altogether, our data show ternary interaction of γ-tubulin with E2F/DP heterodimer and suggest a repressive role for γ-tubulin with E2Fs in controlling mitotic activity and endoreduplication during plant development.

AB - γ-Tubulin is associated with microtubule nucleation, but evidence is accumulating in eukaryotes that it functions also in nuclear processes and in cell division control that are independent of its canonical role. We found that in Arabidopsis thaliana γ-tubulin interacts specifically with E2FA, E2FB, and E2FC transcription factors both in vitro and in vivo. The interaction of γ-tubulin with the E2Fs is not reduced in the presence of the dimerization partners (DPs) and, in agreement, we found that γ-tubulin interaction with E2Fs does not require the dimerization domain. γ-Tubulin associates with the promoters of E2F-regulated cell cycle genes in an E2F dependent manner, likely in complex with the E2F/DP heterodimer. The upregulation of E2F targets; PCNA, ORC2, CDKB1;1 and CCS52A under γ-tubulin silencing suggests a repressive function for γ-tubulin at G1/S, G2/M and endocycle, which is consistent with an excess of cell division in some cells and enhanced endoreduplication in others in the shoot and young leaves of γ-tubulin RNAi plants. Altogether, our data show ternary interaction of γ-tubulin with E2F/DP heterodimer and suggest a repressive role for γ-tubulin with E2Fs in controlling mitotic activity and endoreduplication during plant development.

KW - Arabidopsis, E2F transcription factors, endoreduplication, gene expression, proliferation, γ-Tubulin

U2 - 10.1093/jxb/erz498

DO - 10.1093/jxb/erz498

M3 - Article

JO - Journal of Experimental Botany

JF - Journal of Experimental Botany

SN - 0022-0957

M1 - erz498

ER -