The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis. / Nakabayashi, Kazumi; Walker, Matthew; Irwin, Dianne; Cohn, Jonathan; Guida-English, Stephanie; Garcia, Lucio; Pavlovic, Iva; Novak, Ondrej; Tarkowska, Dana; Strnad, Mirek; Perez Suarez, Marta; Seville, Anne; Stock, David; Leubner-Metzger, Gerhard.

In: International Journal of Molecular Sciences, Vol. 23, No. 9, 4618, 21.04.2022.

Research output: Contribution to journalArticlepeer-review

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The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis. / Nakabayashi, Kazumi; Walker, Matthew; Irwin, Dianne; Cohn, Jonathan; Guida-English, Stephanie; Garcia, Lucio; Pavlovic, Iva; Novak, Ondrej; Tarkowska, Dana; Strnad, Mirek; Perez Suarez, Marta; Seville, Anne; Stock, David; Leubner-Metzger, Gerhard.

In: International Journal of Molecular Sciences, Vol. 23, No. 9, 4618, 21.04.2022.

Research output: Contribution to journalArticlepeer-review

Harvard

Nakabayashi, K, Walker, M, Irwin, D, Cohn, J, Guida-English, S, Garcia, L, Pavlovic, I, Novak, O, Tarkowska, D, Strnad, M, Perez Suarez, M, Seville, A, Stock, D & Leubner-Metzger, G 2022, 'The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis', International Journal of Molecular Sciences, vol. 23, no. 9, 4618. https://doi.org/10.3390/ijms23094618

APA

Nakabayashi, K., Walker, M., Irwin, D., Cohn, J., Guida-English, S., Garcia, L., Pavlovic, I., Novak, O., Tarkowska, D., Strnad, M., Perez Suarez, M., Seville, A., Stock, D., & Leubner-Metzger, G. (2022). The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis. International Journal of Molecular Sciences, 23(9), [4618]. https://doi.org/10.3390/ijms23094618

Vancouver

Author

Nakabayashi, Kazumi ; Walker, Matthew ; Irwin, Dianne ; Cohn, Jonathan ; Guida-English, Stephanie ; Garcia, Lucio ; Pavlovic, Iva ; Novak, Ondrej ; Tarkowska, Dana ; Strnad, Mirek ; Perez Suarez, Marta ; Seville, Anne ; Stock, David ; Leubner-Metzger, Gerhard. / The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis. In: International Journal of Molecular Sciences. 2022 ; Vol. 23, No. 9.

BibTeX

@article{18b90f523ce64bb4be89cde46b431650,
title = "The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis",
abstract = "Molecular responses of plants to natural phytotoxins comprise more general and com-pound-specific mechanisms. How phytotoxic chalcones and other flavonoids inhibit seedling growth was widely studied, but how they interfere with seed germination is largely unknown. The dihydrochalcone and putative allelochemical myrigalone A (MyA) inhibits seed germination and seedling growth. Transcriptome (RNAseq) and hormone analyses of Lepidium sativum seed responses to MyA were compared to other bioactive and inactive compounds. MyA treatment of imbibed seeds triggered the phased induction of a detoxification programme, altered gibberellin, cis-(+)-12-oxophytodienoic acid and jasmonate metabolism, and affected the expression of hor-mone transporter genes. The MyA-mediated inhibition involved interference with the antioxidant system, oxidative signalling, aquaporins and water uptake, but not uncoupling of oxidative phosphorylation or p-hydroxyphenylpyruvate dioxygenase expression/activity. MyA specifically affected the expression of auxin-related signalling genes, and various transporter genes including for auxin transport (PIN7, ABCG37, ABCG4, WAT1). Responses to auxin-specific inhibitors fur-ther supported the conclusion that MyA interferes with auxin homeostasis during seed germina-tion. Comparative analysis of MyA and other phytotoxins revealed differences in the specific reg-ulatory mechanisms and auxin transporter genes targeted to interfere with auxin homestasis. We conclude that MyA exerts its phytotoxic activity by multiple auxin-dependent and independent molecular mechanisms.",
author = "Kazumi Nakabayashi and Matthew Walker and Dianne Irwin and Jonathan Cohn and Stephanie Guida-English and Lucio Garcia and Iva Pavlovic and Ondrej Novak and Dana Tarkowska and Mirek Strnad and {Perez Suarez}, Marta and Anne Seville and David Stock and Gerhard Leubner-Metzger",
year = "2022",
month = apr,
day = "21",
doi = "10.3390/ijms23094618",
language = "English",
volume = "23",
journal = "International Journal of Molecular Sciences",
issn = "1000-9035",
publisher = "MDPI",
number = "9",

}

RIS

TY - JOUR

T1 - The phytotoxin myrigalone A triggers a phased detoxification programme and inhibits Lepidium sativum seed germination via multiple mechanisms including interference with auxin homeostasis

AU - Nakabayashi, Kazumi

AU - Walker, Matthew

AU - Irwin, Dianne

AU - Cohn, Jonathan

AU - Guida-English, Stephanie

AU - Garcia, Lucio

AU - Pavlovic, Iva

AU - Novak, Ondrej

AU - Tarkowska, Dana

AU - Strnad, Mirek

AU - Perez Suarez, Marta

AU - Seville, Anne

AU - Stock, David

AU - Leubner-Metzger, Gerhard

PY - 2022/4/21

Y1 - 2022/4/21

N2 - Molecular responses of plants to natural phytotoxins comprise more general and com-pound-specific mechanisms. How phytotoxic chalcones and other flavonoids inhibit seedling growth was widely studied, but how they interfere with seed germination is largely unknown. The dihydrochalcone and putative allelochemical myrigalone A (MyA) inhibits seed germination and seedling growth. Transcriptome (RNAseq) and hormone analyses of Lepidium sativum seed responses to MyA were compared to other bioactive and inactive compounds. MyA treatment of imbibed seeds triggered the phased induction of a detoxification programme, altered gibberellin, cis-(+)-12-oxophytodienoic acid and jasmonate metabolism, and affected the expression of hor-mone transporter genes. The MyA-mediated inhibition involved interference with the antioxidant system, oxidative signalling, aquaporins and water uptake, but not uncoupling of oxidative phosphorylation or p-hydroxyphenylpyruvate dioxygenase expression/activity. MyA specifically affected the expression of auxin-related signalling genes, and various transporter genes including for auxin transport (PIN7, ABCG37, ABCG4, WAT1). Responses to auxin-specific inhibitors fur-ther supported the conclusion that MyA interferes with auxin homeostasis during seed germina-tion. Comparative analysis of MyA and other phytotoxins revealed differences in the specific reg-ulatory mechanisms and auxin transporter genes targeted to interfere with auxin homestasis. We conclude that MyA exerts its phytotoxic activity by multiple auxin-dependent and independent molecular mechanisms.

AB - Molecular responses of plants to natural phytotoxins comprise more general and com-pound-specific mechanisms. How phytotoxic chalcones and other flavonoids inhibit seedling growth was widely studied, but how they interfere with seed germination is largely unknown. The dihydrochalcone and putative allelochemical myrigalone A (MyA) inhibits seed germination and seedling growth. Transcriptome (RNAseq) and hormone analyses of Lepidium sativum seed responses to MyA were compared to other bioactive and inactive compounds. MyA treatment of imbibed seeds triggered the phased induction of a detoxification programme, altered gibberellin, cis-(+)-12-oxophytodienoic acid and jasmonate metabolism, and affected the expression of hor-mone transporter genes. The MyA-mediated inhibition involved interference with the antioxidant system, oxidative signalling, aquaporins and water uptake, but not uncoupling of oxidative phosphorylation or p-hydroxyphenylpyruvate dioxygenase expression/activity. MyA specifically affected the expression of auxin-related signalling genes, and various transporter genes including for auxin transport (PIN7, ABCG37, ABCG4, WAT1). Responses to auxin-specific inhibitors fur-ther supported the conclusion that MyA interferes with auxin homeostasis during seed germina-tion. Comparative analysis of MyA and other phytotoxins revealed differences in the specific reg-ulatory mechanisms and auxin transporter genes targeted to interfere with auxin homestasis. We conclude that MyA exerts its phytotoxic activity by multiple auxin-dependent and independent molecular mechanisms.

U2 - 10.3390/ijms23094618

DO - 10.3390/ijms23094618

M3 - Article

VL - 23

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1000-9035

IS - 9

M1 - 4618

ER -