The human singing voice changes throughout the lifespan and there are also gender-specific variations that need to be taken into account when working with females. Life changes in terms of voice are different for females and males and this chapter concentrates on the female singing voice in the context of choral singing. Data arepresented relating to the changing female voice during puberty as part of a longitudinal study of female choristers in a major English Cathedral Choir School. In addition, discussion is presented on important considerations with respect to the female choral singing voice in the context of specific choral aspects during rehearsals and performance.