Rocket Science : The Effect of Spaceflight on Germination Physiology, Ageing, and Transcriptome of Eruca sativa Seeds. / Chandler, Jake; Haas, Fabian; Khan, Safina; Bowden, Laura ; Ignatz, Michael; Enfissi, Genny; Gawthrop, Frances; Griffiths, Alistair; Fraser, Paul; Rensing, Stefan; Leubner, Gerhard.

In: Life, Vol. 10, No. 4, 49, 24.04.2020, p. 1-25.

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Rocket Science : The Effect of Spaceflight on Germination Physiology, Ageing, and Transcriptome of Eruca sativa Seeds. / Chandler, Jake; Haas, Fabian; Khan, Safina; Bowden, Laura ; Ignatz, Michael; Enfissi, Genny; Gawthrop, Frances; Griffiths, Alistair; Fraser, Paul; Rensing, Stefan; Leubner, Gerhard.

In: Life, Vol. 10, No. 4, 49, 24.04.2020, p. 1-25.

Research output: Contribution to journalArticle

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@article{31196aaf4bfa4471af9c7f4e9c800c5b,
title = "Rocket Science: The Effect of Spaceflight on Germination Physiology, Ageing, and Transcriptome of Eruca sativa Seeds",
abstract = "In the 'Rocket Science' project storage of Eruca sativa (salad rocket) seeds for six months on board of the International Space Station resulted in delayed seedling establishment. Here we investigated the physiological and molecular mechanisms underpinning the spaceflight effects on dry seeds. We found that 'Space' seed germination vigour was reduced, and ageing sensitivity increased, but the spaceflight did not compromise seed viability and the development of normal seedlings. Comparative analysis of the transcriptomes (using RNAseq) in dry seeds and upon controlled artificial ageing treatment (CAAT) revealed differentially expressed genes (DEGs) associated with spaceflight and ageing. DEG categories enriched by spaceflight and CAAT included transcription and translation with reduced transcript abundances for 40S and 60S ribosomal subunit genes. Among the 'spaceflight-up' DEGs were Heat Shock Protein (HSP), DNAJ-related chaperones, a Heat Shock Factor (HSFA7a-like), and components of several DNA repair pathways (e.g. ATM, DNA ligase1). The 'response to radiation' category was especially enriched in 'spaceflight-up' DEGs including HSPs, catalases and the transcription factor HY5. The major finding from the physiological and transcriptome analysis is that spaceflight causes vigour loss and partial ageing during air-dry seed storage, for which space environmental factors and consequences for seed storage during spaceflights are discussed.",
keywords = "Climate change, food security, Low Earth Orbit, salad rocket (Eruca sativa), seed germination, spaceflight transcriptomes, seed ageing, seeds in Space, seed storage, seed vigour",
author = "Jake Chandler and Fabian Haas and Safina Khan and Laura Bowden and Michael Ignatz and Genny Enfissi and Frances Gawthrop and Alistair Griffiths and Paul Fraser and Stefan Rensing and Gerhard Leubner",
note = "Special Issue {"}Frontiers in Astrobiology{"}",
year = "2020",
month = apr,
day = "24",
doi = "10.3390/life10040049",
language = "English",
volume = "10",
pages = "1--25",
journal = "Life",
issn = "2075-1729",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "4",

}

RIS

TY - JOUR

T1 - Rocket Science

T2 - The Effect of Spaceflight on Germination Physiology, Ageing, and Transcriptome of Eruca sativa Seeds

AU - Chandler, Jake

AU - Haas, Fabian

AU - Khan, Safina

AU - Bowden, Laura

AU - Ignatz, Michael

AU - Enfissi, Genny

AU - Gawthrop, Frances

AU - Griffiths, Alistair

AU - Fraser, Paul

AU - Rensing, Stefan

AU - Leubner, Gerhard

N1 - Special Issue "Frontiers in Astrobiology"

PY - 2020/4/24

Y1 - 2020/4/24

N2 - In the 'Rocket Science' project storage of Eruca sativa (salad rocket) seeds for six months on board of the International Space Station resulted in delayed seedling establishment. Here we investigated the physiological and molecular mechanisms underpinning the spaceflight effects on dry seeds. We found that 'Space' seed germination vigour was reduced, and ageing sensitivity increased, but the spaceflight did not compromise seed viability and the development of normal seedlings. Comparative analysis of the transcriptomes (using RNAseq) in dry seeds and upon controlled artificial ageing treatment (CAAT) revealed differentially expressed genes (DEGs) associated with spaceflight and ageing. DEG categories enriched by spaceflight and CAAT included transcription and translation with reduced transcript abundances for 40S and 60S ribosomal subunit genes. Among the 'spaceflight-up' DEGs were Heat Shock Protein (HSP), DNAJ-related chaperones, a Heat Shock Factor (HSFA7a-like), and components of several DNA repair pathways (e.g. ATM, DNA ligase1). The 'response to radiation' category was especially enriched in 'spaceflight-up' DEGs including HSPs, catalases and the transcription factor HY5. The major finding from the physiological and transcriptome analysis is that spaceflight causes vigour loss and partial ageing during air-dry seed storage, for which space environmental factors and consequences for seed storage during spaceflights are discussed.

AB - In the 'Rocket Science' project storage of Eruca sativa (salad rocket) seeds for six months on board of the International Space Station resulted in delayed seedling establishment. Here we investigated the physiological and molecular mechanisms underpinning the spaceflight effects on dry seeds. We found that 'Space' seed germination vigour was reduced, and ageing sensitivity increased, but the spaceflight did not compromise seed viability and the development of normal seedlings. Comparative analysis of the transcriptomes (using RNAseq) in dry seeds and upon controlled artificial ageing treatment (CAAT) revealed differentially expressed genes (DEGs) associated with spaceflight and ageing. DEG categories enriched by spaceflight and CAAT included transcription and translation with reduced transcript abundances for 40S and 60S ribosomal subunit genes. Among the 'spaceflight-up' DEGs were Heat Shock Protein (HSP), DNAJ-related chaperones, a Heat Shock Factor (HSFA7a-like), and components of several DNA repair pathways (e.g. ATM, DNA ligase1). The 'response to radiation' category was especially enriched in 'spaceflight-up' DEGs including HSPs, catalases and the transcription factor HY5. The major finding from the physiological and transcriptome analysis is that spaceflight causes vigour loss and partial ageing during air-dry seed storage, for which space environmental factors and consequences for seed storage during spaceflights are discussed.

KW - Climate change

KW - food security

KW - Low Earth Orbit

KW - salad rocket (Eruca sativa)

KW - seed germination

KW - spaceflight transcriptomes

KW - seed ageing

KW - seeds in Space

KW - seed storage

KW - seed vigour

U2 - 10.3390/life10040049

DO - 10.3390/life10040049

M3 - Article

VL - 10

SP - 1

EP - 25

JO - Life

JF - Life

SN - 2075-1729

IS - 4

M1 - 49

ER -