Properties of Nicotiana glauca as a biorefining feedstock. / Kozlo, Amanda.

Royal Holloway, 2017. 287 p.

Research output: ThesisDoctoral Thesis

Unpublished

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Properties of Nicotiana glauca as a biorefining feedstock. / Kozlo, Amanda.

Royal Holloway, 2017. 287 p.

Research output: ThesisDoctoral Thesis

Harvard

Kozlo, A 2017, 'Properties of Nicotiana glauca as a biorefining feedstock', Ph.D., Royal Holloway, University of London.

APA

Vancouver

Author

BibTeX

@phdthesis{418294cf45f644e7b69c2f801e2ac081,
title = "Properties of Nicotiana glauca as a biorefining feedstock",
abstract = "Increasing issues of climate change and land availability/allocation for food and feedhave been pressurising world societies to advance environmental sustainability. Becauseof that, the biorefining concept evolved to replace petro–based industries, while thesustainability focuses on the society, economy and the environmental issues raised as partof the renewable energy policies. Plant–fuelled biorefinery have potential to becoming agreat alternative to fossil fuels, however the competition with the food market is strainingthe supply of food and its prices, therefore needs to be addressed immediately.Nicotiana glauca, otherwise called Tree tobacco is a potential source for biorefinery thatdoes not compete with the food market unlike other plant based sources currently used.A globally naturalised South American shrub, it is a fast growing and well habituatedshrub in dry and arid areas in countries such as USA, Australia, Asian and most southernEuropean countries yet is still comparable to the traditional crops used for biorefinery. Alarge above–ground biomass shrub featuring sticky and rubbery pale surface and thickleaves with grey appearance is a self fertile, amenable to genetic manipulation and fastgrowing plant. In the present study it was evaluated for its potential to become integratedinto current renewable source economy as a feedstock to replace petro–chemical refinery.System biology tools were utilised encompassing non–polar, polar and isoprenoid metaboliteanalysis to assess the changes of the metabolite profile over leaf development as wellas to identify the levels of high–value compounds naturally occurring in Nicotiana glauca.The leaves were found to contain a simple metabolite profile rich in fatty acids (35% oftotal non–polar dry leaf material), carotenoids and other high value compounds includingeasily extractable leaf surface containing high amounts of pure long chain hydrocarbons(C29– C33, at 96%). A number of compounds have been identified to be potentially usedto produce for e.g. bioplastics, organic solvents and biodiesel. The study also evaluatedthe metabolite profile of a ketocarotenoid engineered line containing crt hydroxylaseand ketolase genes. The evaluation of increasing value through the occurring metabolitechanges showed the patterns underlying a plausible connection between the fattyacid and carotenoid biosynthetic pathways in the crop. As a result, the thesis coveredindustrial application of the crop and characterised biochemical roles between the twopathways.",
keywords = "Nicotiana glauca, Ketocarotenoids, Lipids, Lipid Metabolism, crtz/w, feedstock, Biorefining, Metabolic profiling, Amanda Kozlo, Kozlo, PhD",
author = "Amanda Kozlo",
year = "2017",
language = "English",
publisher = "Royal Holloway",
school = "Royal Holloway, University of London",

}

RIS

TY - THES

T1 - Properties of Nicotiana glauca as a biorefining feedstock

AU - Kozlo, Amanda

PY - 2017

Y1 - 2017

N2 - Increasing issues of climate change and land availability/allocation for food and feedhave been pressurising world societies to advance environmental sustainability. Becauseof that, the biorefining concept evolved to replace petro–based industries, while thesustainability focuses on the society, economy and the environmental issues raised as partof the renewable energy policies. Plant–fuelled biorefinery have potential to becoming agreat alternative to fossil fuels, however the competition with the food market is strainingthe supply of food and its prices, therefore needs to be addressed immediately.Nicotiana glauca, otherwise called Tree tobacco is a potential source for biorefinery thatdoes not compete with the food market unlike other plant based sources currently used.A globally naturalised South American shrub, it is a fast growing and well habituatedshrub in dry and arid areas in countries such as USA, Australia, Asian and most southernEuropean countries yet is still comparable to the traditional crops used for biorefinery. Alarge above–ground biomass shrub featuring sticky and rubbery pale surface and thickleaves with grey appearance is a self fertile, amenable to genetic manipulation and fastgrowing plant. In the present study it was evaluated for its potential to become integratedinto current renewable source economy as a feedstock to replace petro–chemical refinery.System biology tools were utilised encompassing non–polar, polar and isoprenoid metaboliteanalysis to assess the changes of the metabolite profile over leaf development as wellas to identify the levels of high–value compounds naturally occurring in Nicotiana glauca.The leaves were found to contain a simple metabolite profile rich in fatty acids (35% oftotal non–polar dry leaf material), carotenoids and other high value compounds includingeasily extractable leaf surface containing high amounts of pure long chain hydrocarbons(C29– C33, at 96%). A number of compounds have been identified to be potentially usedto produce for e.g. bioplastics, organic solvents and biodiesel. The study also evaluatedthe metabolite profile of a ketocarotenoid engineered line containing crt hydroxylaseand ketolase genes. The evaluation of increasing value through the occurring metabolitechanges showed the patterns underlying a plausible connection between the fattyacid and carotenoid biosynthetic pathways in the crop. As a result, the thesis coveredindustrial application of the crop and characterised biochemical roles between the twopathways.

AB - Increasing issues of climate change and land availability/allocation for food and feedhave been pressurising world societies to advance environmental sustainability. Becauseof that, the biorefining concept evolved to replace petro–based industries, while thesustainability focuses on the society, economy and the environmental issues raised as partof the renewable energy policies. Plant–fuelled biorefinery have potential to becoming agreat alternative to fossil fuels, however the competition with the food market is strainingthe supply of food and its prices, therefore needs to be addressed immediately.Nicotiana glauca, otherwise called Tree tobacco is a potential source for biorefinery thatdoes not compete with the food market unlike other plant based sources currently used.A globally naturalised South American shrub, it is a fast growing and well habituatedshrub in dry and arid areas in countries such as USA, Australia, Asian and most southernEuropean countries yet is still comparable to the traditional crops used for biorefinery. Alarge above–ground biomass shrub featuring sticky and rubbery pale surface and thickleaves with grey appearance is a self fertile, amenable to genetic manipulation and fastgrowing plant. In the present study it was evaluated for its potential to become integratedinto current renewable source economy as a feedstock to replace petro–chemical refinery.System biology tools were utilised encompassing non–polar, polar and isoprenoid metaboliteanalysis to assess the changes of the metabolite profile over leaf development as wellas to identify the levels of high–value compounds naturally occurring in Nicotiana glauca.The leaves were found to contain a simple metabolite profile rich in fatty acids (35% oftotal non–polar dry leaf material), carotenoids and other high value compounds includingeasily extractable leaf surface containing high amounts of pure long chain hydrocarbons(C29– C33, at 96%). A number of compounds have been identified to be potentially usedto produce for e.g. bioplastics, organic solvents and biodiesel. The study also evaluatedthe metabolite profile of a ketocarotenoid engineered line containing crt hydroxylaseand ketolase genes. The evaluation of increasing value through the occurring metabolitechanges showed the patterns underlying a plausible connection between the fattyacid and carotenoid biosynthetic pathways in the crop. As a result, the thesis coveredindustrial application of the crop and characterised biochemical roles between the twopathways.

KW - Nicotiana glauca

KW - Ketocarotenoids

KW - Lipids

KW - Lipid Metabolism

KW - crtz/w

KW - feedstock

KW - Biorefining

KW - Metabolic profiling

KW - Amanda Kozlo

KW - Kozlo

KW - PhD

M3 - Doctoral Thesis

PB - Royal Holloway

ER -