Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases. / Scheler, Claudia; Weitbrecht, Karin; Pearce, Simon; Hampstead, Anthony; Büttner-Mainik, A; Lee, Kieran JD; Voegele, Antje; Oracz, Krystyna; Dekkers, Bas JW; Wang, Xiaofeng; Wood, Andy; Bentsink, Leonie; King, John R.; Knox, Paul; Holdsworth, Mike; Müller, Kerstin; Leubner-Metzger, Gerhard.

In: Plant physiology, Vol. 167, No. 1, 01.2015, p. 200-215.

Research output: Contribution to journalArticlepeer-review

Published

Standard

Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases. / Scheler, Claudia; Weitbrecht, Karin; Pearce, Simon; Hampstead, Anthony; Büttner-Mainik, A; Lee, Kieran JD; Voegele, Antje; Oracz, Krystyna; Dekkers, Bas JW; Wang, Xiaofeng; Wood, Andy; Bentsink, Leonie; King, John R.; Knox, Paul; Holdsworth, Mike; Müller, Kerstin; Leubner-Metzger, Gerhard.

In: Plant physiology, Vol. 167, No. 1, 01.2015, p. 200-215.

Research output: Contribution to journalArticlepeer-review

Harvard

Scheler, C, Weitbrecht, K, Pearce, S, Hampstead, A, Büttner-Mainik, A, Lee, KJD, Voegele, A, Oracz, K, Dekkers, BJW, Wang, X, Wood, A, Bentsink, L, King, JR, Knox, P, Holdsworth, M, Müller, K & Leubner-Metzger, G 2015, 'Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases', Plant physiology, vol. 167, no. 1, pp. 200-215. https://doi.org/10.1104/pp.114.247429

APA

Scheler, C., Weitbrecht, K., Pearce, S., Hampstead, A., Büttner-Mainik, A., Lee, K. JD., Voegele, A., Oracz, K., Dekkers, B. JW., Wang, X., Wood, A., Bentsink, L., King, J. R., Knox, P., Holdsworth, M., Müller, K., & Leubner-Metzger, G. (2015). Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases. Plant physiology, 167(1), 200-215. https://doi.org/10.1104/pp.114.247429

Vancouver

Scheler C, Weitbrecht K, Pearce S, Hampstead A, Büttner-Mainik A, Lee KJD et al. Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases. Plant physiology. 2015 Jan;167(1):200-215. https://doi.org/10.1104/pp.114.247429

Author

Scheler, Claudia ; Weitbrecht, Karin ; Pearce, Simon ; Hampstead, Anthony ; Büttner-Mainik, A ; Lee, Kieran JD ; Voegele, Antje ; Oracz, Krystyna ; Dekkers, Bas JW ; Wang, Xiaofeng ; Wood, Andy ; Bentsink, Leonie ; King, John R. ; Knox, Paul ; Holdsworth, Mike ; Müller, Kerstin ; Leubner-Metzger, Gerhard. / Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases. In: Plant physiology. 2015 ; Vol. 167, No. 1. pp. 200-215.

BibTeX

@article{cb710f5131554e7991937cd15891d4b5,
title = "Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases",
abstract = "Pectin methylesterase (PME) controls the methylesterification status of pectins and thereby determines the biophysical properties of plant cell walls, which are important for tissue growth and weakening processes. We demonstrate here that tissue-specific and spatio-temporal alterations in cell wall pectin methylesterification occur during the germination of Lepidium sativum. These cell wall changes are associated with characteristic expression patterns of PME genes and resultant enzyme activities in the key seed compartments CAP (micropylar endosperm) and RAD (radicle plus lower hypocotyl). Transcriptome and RT-qPCR analysis as well as PME enzyme activity measurements of separated seed compartments, including CAP and RAD, revealed distinct phases during germination. These were associated with hormonal and compartment-specific regulation of PME group 1, PME group 2, and PME inhibitor (PMEI) transcript expression and total PME activity. The regulatory patterns indicated a role for PME activity in testa rupture (TR). Consistent with a role for cell wall pectin methylesterification in TR, treatment of seeds with PME resulted in enhanced testa permeability and promoted TR. Mathematical modelling of transcript expression changes in germinating L. sativum and Arabidopsis thaliana seeds suggested that group 2 PMEs make a major contribution to the overall PME activity rather than acting as PME inhibitors. It is concluded that regulated changes in the degree of pectin methylesterification through CAP and RAD specific PME and PMEI expression play a crucial role during Brassicaceae seed germination.",
keywords = "Seed Pectin Methylesterases, Seed Germination, Lepidium sativum, Cell wall pectins, Testa rupture, Endosperm rupture, Seed coat permeability, Transcriptome",
author = "Claudia Scheler and Karin Weitbrecht and Simon Pearce and Anthony Hampstead and A B{\"u}ttner-Mainik and Lee, {Kieran JD} and Antje Voegele and Krystyna Oracz and Dekkers, {Bas JW} and Xiaofeng Wang and Andy Wood and Leonie Bentsink and King, {John R.} and Paul Knox and Mike Holdsworth and Kerstin M{\"u}ller and Gerhard Leubner-Metzger",
year = "2015",
month = jan,
doi = "10.1104/pp.114.247429",
language = "English",
volume = "167",
pages = "200--215",
journal = "Plant physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "1",

}

RIS

TY - JOUR

T1 - Promotion of Testa Rupture during Garden Cress Germination Involves Seed Compartment-Specific Expression and Activity of Pectin Methylesterases

AU - Scheler, Claudia

AU - Weitbrecht, Karin

AU - Pearce, Simon

AU - Hampstead, Anthony

AU - Büttner-Mainik, A

AU - Lee, Kieran JD

AU - Voegele, Antje

AU - Oracz, Krystyna

AU - Dekkers, Bas JW

AU - Wang, Xiaofeng

AU - Wood, Andy

AU - Bentsink, Leonie

AU - King, John R.

AU - Knox, Paul

AU - Holdsworth, Mike

AU - Müller, Kerstin

AU - Leubner-Metzger, Gerhard

PY - 2015/1

Y1 - 2015/1

N2 - Pectin methylesterase (PME) controls the methylesterification status of pectins and thereby determines the biophysical properties of plant cell walls, which are important for tissue growth and weakening processes. We demonstrate here that tissue-specific and spatio-temporal alterations in cell wall pectin methylesterification occur during the germination of Lepidium sativum. These cell wall changes are associated with characteristic expression patterns of PME genes and resultant enzyme activities in the key seed compartments CAP (micropylar endosperm) and RAD (radicle plus lower hypocotyl). Transcriptome and RT-qPCR analysis as well as PME enzyme activity measurements of separated seed compartments, including CAP and RAD, revealed distinct phases during germination. These were associated with hormonal and compartment-specific regulation of PME group 1, PME group 2, and PME inhibitor (PMEI) transcript expression and total PME activity. The regulatory patterns indicated a role for PME activity in testa rupture (TR). Consistent with a role for cell wall pectin methylesterification in TR, treatment of seeds with PME resulted in enhanced testa permeability and promoted TR. Mathematical modelling of transcript expression changes in germinating L. sativum and Arabidopsis thaliana seeds suggested that group 2 PMEs make a major contribution to the overall PME activity rather than acting as PME inhibitors. It is concluded that regulated changes in the degree of pectin methylesterification through CAP and RAD specific PME and PMEI expression play a crucial role during Brassicaceae seed germination.

AB - Pectin methylesterase (PME) controls the methylesterification status of pectins and thereby determines the biophysical properties of plant cell walls, which are important for tissue growth and weakening processes. We demonstrate here that tissue-specific and spatio-temporal alterations in cell wall pectin methylesterification occur during the germination of Lepidium sativum. These cell wall changes are associated with characteristic expression patterns of PME genes and resultant enzyme activities in the key seed compartments CAP (micropylar endosperm) and RAD (radicle plus lower hypocotyl). Transcriptome and RT-qPCR analysis as well as PME enzyme activity measurements of separated seed compartments, including CAP and RAD, revealed distinct phases during germination. These were associated with hormonal and compartment-specific regulation of PME group 1, PME group 2, and PME inhibitor (PMEI) transcript expression and total PME activity. The regulatory patterns indicated a role for PME activity in testa rupture (TR). Consistent with a role for cell wall pectin methylesterification in TR, treatment of seeds with PME resulted in enhanced testa permeability and promoted TR. Mathematical modelling of transcript expression changes in germinating L. sativum and Arabidopsis thaliana seeds suggested that group 2 PMEs make a major contribution to the overall PME activity rather than acting as PME inhibitors. It is concluded that regulated changes in the degree of pectin methylesterification through CAP and RAD specific PME and PMEI expression play a crucial role during Brassicaceae seed germination.

KW - Seed Pectin Methylesterases

KW - Seed Germination

KW - Lepidium sativum

KW - Cell wall pectins

KW - Testa rupture

KW - Endosperm rupture

KW - Seed coat permeability

KW - Transcriptome

U2 - 10.1104/pp.114.247429

DO - 10.1104/pp.114.247429

M3 - Article

VL - 167

SP - 200

EP - 215

JO - Plant physiology

JF - Plant physiology

SN - 0032-0889

IS - 1

ER -