Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids. / Mortimer, Cara; Misawa, Norihiko ; Ducreux, Laurence ; Campbell, Raymond ; Bramley, Peter; Taylor, Mark; Fraser, Paul.

In: Plant biotechnology journal, Vol. 14, No. 1, 01.2016, p. 140-152.

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Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids. / Mortimer, Cara; Misawa, Norihiko ; Ducreux, Laurence ; Campbell, Raymond ; Bramley, Peter; Taylor, Mark; Fraser, Paul.

In: Plant biotechnology journal, Vol. 14, No. 1, 01.2016, p. 140-152.

Research output: Contribution to journalArticlepeer-review

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Mortimer, Cara ; Misawa, Norihiko ; Ducreux, Laurence ; Campbell, Raymond ; Bramley, Peter ; Taylor, Mark ; Fraser, Paul. / Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids. In: Plant biotechnology journal. 2016 ; Vol. 14, No. 1. pp. 140-152.

BibTeX

@article{7d830e4dffbd453d9242260a0788a4b7,
title = "Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids",
abstract = "To produce commercially valuable ketocarotenoids in Solanum tuberosum, the 4, 4′ β-oxygenase (crtW) and 3, 3′ β-hydroxylase (crtZ) genes from Brevundimonas spp. have been expressed in the plant host under constitutive transcriptional control. The CRTW and CRTZ enzymes are capable of modifying endogenous plant carotenoids to form a range of hydroxylated and ketolated derivatives. The host (cv. D{\'e}sir{\'e}e) produced significant levels of nonendogenous carotenoid products in all tissues, but at the apparent expense of the economically critical metabolite, starch. Carotenoid levels increased in both wild-type and transgenic tubers following cold storage; however, stability during heat processing varied between compounds. Subcellular fractionation of leaf tissues revealed the presence of ketocarotenoids in thylakoid membranes, but not predominantly in the photosynthetic complexes. A dramatic increase in the carotenoid content of plastoglobuli was determined. These findings were corroborated by microscopic analysis of chloroplasts. In tuber tissues, esterified carotenoids, representing 13% of the total pigment found in wild-type extracts, were sequestered in plastoglobuli. In the transgenic tubers, this proportion increased to 45%, with esterified nonendogenous carotenoids in place of endogenous compounds. Conversely, nonesterified carotenoids in both wild-type and transgenic tuber tissues were associated with amyloplast membranes and starch granules.",
keywords = "ketocarotenoid, sequestration, metabolic engineering, Solanum tuberosum, starch",
author = "Cara Mortimer and Norihiko Misawa and Laurence Ducreux and Raymond Campbell and Peter Bramley and Mark Taylor and Paul Fraser",
year = "2016",
month = jan,
doi = "10.1111/pbi.12365",
language = "English",
volume = "14",
pages = "140--152",
journal = "Plant biotechnology journal",
issn = "1860-6768",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Product stability and sequestration mechanisms in Solanum tuberosum engineered to biosynthesize high value ketocarotenoids

AU - Mortimer, Cara

AU - Misawa, Norihiko

AU - Ducreux, Laurence

AU - Campbell, Raymond

AU - Bramley, Peter

AU - Taylor, Mark

AU - Fraser, Paul

PY - 2016/1

Y1 - 2016/1

N2 - To produce commercially valuable ketocarotenoids in Solanum tuberosum, the 4, 4′ β-oxygenase (crtW) and 3, 3′ β-hydroxylase (crtZ) genes from Brevundimonas spp. have been expressed in the plant host under constitutive transcriptional control. The CRTW and CRTZ enzymes are capable of modifying endogenous plant carotenoids to form a range of hydroxylated and ketolated derivatives. The host (cv. Désirée) produced significant levels of nonendogenous carotenoid products in all tissues, but at the apparent expense of the economically critical metabolite, starch. Carotenoid levels increased in both wild-type and transgenic tubers following cold storage; however, stability during heat processing varied between compounds. Subcellular fractionation of leaf tissues revealed the presence of ketocarotenoids in thylakoid membranes, but not predominantly in the photosynthetic complexes. A dramatic increase in the carotenoid content of plastoglobuli was determined. These findings were corroborated by microscopic analysis of chloroplasts. In tuber tissues, esterified carotenoids, representing 13% of the total pigment found in wild-type extracts, were sequestered in plastoglobuli. In the transgenic tubers, this proportion increased to 45%, with esterified nonendogenous carotenoids in place of endogenous compounds. Conversely, nonesterified carotenoids in both wild-type and transgenic tuber tissues were associated with amyloplast membranes and starch granules.

AB - To produce commercially valuable ketocarotenoids in Solanum tuberosum, the 4, 4′ β-oxygenase (crtW) and 3, 3′ β-hydroxylase (crtZ) genes from Brevundimonas spp. have been expressed in the plant host under constitutive transcriptional control. The CRTW and CRTZ enzymes are capable of modifying endogenous plant carotenoids to form a range of hydroxylated and ketolated derivatives. The host (cv. Désirée) produced significant levels of nonendogenous carotenoid products in all tissues, but at the apparent expense of the economically critical metabolite, starch. Carotenoid levels increased in both wild-type and transgenic tubers following cold storage; however, stability during heat processing varied between compounds. Subcellular fractionation of leaf tissues revealed the presence of ketocarotenoids in thylakoid membranes, but not predominantly in the photosynthetic complexes. A dramatic increase in the carotenoid content of plastoglobuli was determined. These findings were corroborated by microscopic analysis of chloroplasts. In tuber tissues, esterified carotenoids, representing 13% of the total pigment found in wild-type extracts, were sequestered in plastoglobuli. In the transgenic tubers, this proportion increased to 45%, with esterified nonendogenous carotenoids in place of endogenous compounds. Conversely, nonesterified carotenoids in both wild-type and transgenic tuber tissues were associated with amyloplast membranes and starch granules.

KW - ketocarotenoid, sequestration, metabolic engineering, Solanum tuberosum, starch

U2 - 10.1111/pbi.12365

DO - 10.1111/pbi.12365

M3 - Article

VL - 14

SP - 140

EP - 152

JO - Plant biotechnology journal

JF - Plant biotechnology journal

SN - 1860-6768

IS - 1

ER -