Peptides and Anti-peptide Antibodies for Small and Medium Scale Peptide and Anti-peptide Affinity Microarrays. / Zhang, Fan; Cleaver, Sophie; Gardner, Matthew; Briones, Andrea; Soloviev, Mikhail.

Peptide Microarrays : Methods and Protocols. Vol. (In press) 3rd Edition. ed. United States : Springer-Verlag New York Inc., 2022. (Methods in Molecular Biology).

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Abstract

This chapter describes the principles for selection of antigenic peptides for development of anti-peptide antibodies suitable for microarray-based multiplex affinity assays and optional mass-spectrometry detection. The methods described here are mostly applicable to small and medium scale multiplex affinity assay and microarrays. Although the same principles of peptide selection may also be applied to larger scale arrays (with 100+ features), informatics software and printing methods may well differ. Due to the sheer number of proteins/peptides to be processed and analyzed, dedicated software with high processing capacity and enterprise level array robotics may be required for larger scale efforts. This report aims to provide practical advice to those seeking to develop or use arrays with up to ~100 different peptide or protein features.
Original languageEnglish
Title of host publicationPeptide Microarrays : Methods and Protocols
Place of PublicationUnited States
PublisherSpringer-Verlag New York Inc.
Volume(In press)
Edition3rd Edition
ISBN (Print)1071627317
Publication statusPublished - 8 Oct 2022

Publication series

NameMethods in Molecular Biology

ID: 45881811