Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells. / Dickson, G; Prentice, H; Julien, J P; Ferrari, G; Leon, A; Walsh, F S.

In: EMBO Journal , Vol. 5, No. 13, 1986, p. 3449-53.

Research output: Contribution to journalArticlepeer-review

Published

Standard

Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells. / Dickson, G; Prentice, H; Julien, J P; Ferrari, G; Leon, A; Walsh, F S.

In: EMBO Journal , Vol. 5, No. 13, 1986, p. 3449-53.

Research output: Contribution to journalArticlepeer-review

Harvard

Dickson, G, Prentice, H, Julien, JP, Ferrari, G, Leon, A & Walsh, FS 1986, 'Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells', EMBO Journal , vol. 5, no. 13, pp. 3449-53.

APA

Dickson, G., Prentice, H., Julien, J. P., Ferrari, G., Leon, A., & Walsh, F. S. (1986). Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells. EMBO Journal , 5(13), 3449-53.

Vancouver

Dickson G, Prentice H, Julien JP, Ferrari G, Leon A, Walsh FS. Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells. EMBO Journal . 1986;5(13):3449-53.

Author

Dickson, G ; Prentice, H ; Julien, J P ; Ferrari, G ; Leon, A ; Walsh, F S. / Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells. In: EMBO Journal . 1986 ; Vol. 5, No. 13. pp. 3449-53.

BibTeX

@article{1d4c145d063f4b63b7f6d361344cb64c,
title = "Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells",
abstract = "The effect of nerve growth factor (NGF) on the expression of neurofilament and Thy-1 genes in rat PC12 pheochromocytoma cells was examined at both the transcriptional and post-transcriptional levels. Addition of NGF to cultured PC12 cells produced increases in mRNAs corresponding to the 68 kd neurofilament protein (NF68) and the Thy-1 glycoprotein within 24 h, with maximal effects of some 90- and 45-fold stimulation (relative to beta-actin mRNA) being observed after 12 and 4 days of treatment, respectively. In addition, transcriptional run-off analyses using isolated nuclei showed that NGF treatment resulted directly in 8- and 4-fold increases in the rate of NF68 and Thy-1 gene transcription. These gene activation events were independent of overt morphological differentiation of PC12 cells occurring both under conditions permissive and non-permissive for neurite outgrowth, and once established the new molecular phenotype was dependent upon the continued presence of NGF. This is the first molecular evidence for the reversible activation of neuron-specific genes during NGF-induced differentiation in PC12 cells.",
author = "G Dickson and H Prentice and Julien, {J P} and G Ferrari and A Leon and Walsh, {F S}",
year = "1986",
language = "English",
volume = "5",
pages = "3449--53",
journal = "EMBO Journal ",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "13",

}

RIS

TY - JOUR

T1 - Nerve growth factor activates Thy-1 and neurofilament gene transcription in rat PC12 cells

AU - Dickson, G

AU - Prentice, H

AU - Julien, J P

AU - Ferrari, G

AU - Leon, A

AU - Walsh, F S

PY - 1986

Y1 - 1986

N2 - The effect of nerve growth factor (NGF) on the expression of neurofilament and Thy-1 genes in rat PC12 pheochromocytoma cells was examined at both the transcriptional and post-transcriptional levels. Addition of NGF to cultured PC12 cells produced increases in mRNAs corresponding to the 68 kd neurofilament protein (NF68) and the Thy-1 glycoprotein within 24 h, with maximal effects of some 90- and 45-fold stimulation (relative to beta-actin mRNA) being observed after 12 and 4 days of treatment, respectively. In addition, transcriptional run-off analyses using isolated nuclei showed that NGF treatment resulted directly in 8- and 4-fold increases in the rate of NF68 and Thy-1 gene transcription. These gene activation events were independent of overt morphological differentiation of PC12 cells occurring both under conditions permissive and non-permissive for neurite outgrowth, and once established the new molecular phenotype was dependent upon the continued presence of NGF. This is the first molecular evidence for the reversible activation of neuron-specific genes during NGF-induced differentiation in PC12 cells.

AB - The effect of nerve growth factor (NGF) on the expression of neurofilament and Thy-1 genes in rat PC12 pheochromocytoma cells was examined at both the transcriptional and post-transcriptional levels. Addition of NGF to cultured PC12 cells produced increases in mRNAs corresponding to the 68 kd neurofilament protein (NF68) and the Thy-1 glycoprotein within 24 h, with maximal effects of some 90- and 45-fold stimulation (relative to beta-actin mRNA) being observed after 12 and 4 days of treatment, respectively. In addition, transcriptional run-off analyses using isolated nuclei showed that NGF treatment resulted directly in 8- and 4-fold increases in the rate of NF68 and Thy-1 gene transcription. These gene activation events were independent of overt morphological differentiation of PC12 cells occurring both under conditions permissive and non-permissive for neurite outgrowth, and once established the new molecular phenotype was dependent upon the continued presence of NGF. This is the first molecular evidence for the reversible activation of neuron-specific genes during NGF-induced differentiation in PC12 cells.

M3 - Article

C2 - 16453742

VL - 5

SP - 3449

EP - 3453

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 13

ER -