Molecular mechanisms and hormonal regulation underpinning morphological dormancy : a case study using Apium graveolens (Apiaceae). / Walker, Matthew; Perez Suarez, Marta; Steinbrecher, Tina; Gawthrop, Frances; Pavlovic, Iva; Novak, Ondrej; Tarkowska, Dana; Strnad, Mirek; Marone, Federica; Nakabayashi, Kazumi; Leubner-Metzger, Gerhard.

In: Plant Journal, 12.09.2021.

Research output: Contribution to journalArticlepeer-review

E-pub ahead of print

Standard

Molecular mechanisms and hormonal regulation underpinning morphological dormancy : a case study using Apium graveolens (Apiaceae). / Walker, Matthew; Perez Suarez, Marta; Steinbrecher, Tina; Gawthrop, Frances; Pavlovic, Iva; Novak, Ondrej; Tarkowska, Dana; Strnad, Mirek; Marone, Federica; Nakabayashi, Kazumi; Leubner-Metzger, Gerhard.

In: Plant Journal, 12.09.2021.

Research output: Contribution to journalArticlepeer-review

Harvard

APA

Vancouver

Author

BibTeX

@article{15e1caee6cd1498c80b13628e338e7b3,
title = "Molecular mechanisms and hormonal regulation underpinning morphological dormancy: a case study using Apium graveolens (Apiaceae)",
abstract = "Underdeveloped (small) embryos embedded in abundant endosperm tissue, and thus having morphological (MD) or morphophysiological (MPD) dormancy, are considered to be the ancestral state in seed dormancy evolution. This trait is retained in the Apiaceae family which provides excellent model systems to study the underpinning mechanisms. We investigated Apium graveolens (celery) MD by combined innovative imaging and embryo growth assays with the quantification of hormone metabolism, as well as the analysis of hormone and cell-wall related gene expression. The integrated experimental results demonstrated that embryo growth occurred inside imbibed celery fruits in association with endosperm degradation, and that a critical embryo size was required for radicle emergence. The regulation of these processes depends on gene expression leading to gibberellin and indole-3-acetic acid (IAA) production by the embryo and on crosstalk between the fruit compartments. Abscisic acid (ABA) degradation associated with distinct spatiotemporal patterns in ABA sensitivity control embryo growth, endosperm breakdown and radicle emergence. This complex interaction between gibberellins, IAA and ABA metabolism, and changes in the tissue-specific sensitivities to these hormones is distinct from non-MD seeds. We conclude that the embryo growth to reach the critical size and the associated endosperm breakdown inside MD fruits constitute a unique germination programme.",
author = "Matthew Walker and {Perez Suarez}, Marta and Tina Steinbrecher and Frances Gawthrop and Iva Pavlovic and Ondrej Novak and Dana Tarkowska and Mirek Strnad and Federica Marone and Kazumi Nakabayashi and Gerhard Leubner-Metzger",
year = "2021",
month = sep,
day = "12",
doi = "10.1111/tpj.15489",
language = "English",
journal = "Plant Journal",
issn = "0960-7412",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - Molecular mechanisms and hormonal regulation underpinning morphological dormancy

T2 - a case study using Apium graveolens (Apiaceae)

AU - Walker, Matthew

AU - Perez Suarez, Marta

AU - Steinbrecher, Tina

AU - Gawthrop, Frances

AU - Pavlovic, Iva

AU - Novak, Ondrej

AU - Tarkowska, Dana

AU - Strnad, Mirek

AU - Marone, Federica

AU - Nakabayashi, Kazumi

AU - Leubner-Metzger, Gerhard

PY - 2021/9/12

Y1 - 2021/9/12

N2 - Underdeveloped (small) embryos embedded in abundant endosperm tissue, and thus having morphological (MD) or morphophysiological (MPD) dormancy, are considered to be the ancestral state in seed dormancy evolution. This trait is retained in the Apiaceae family which provides excellent model systems to study the underpinning mechanisms. We investigated Apium graveolens (celery) MD by combined innovative imaging and embryo growth assays with the quantification of hormone metabolism, as well as the analysis of hormone and cell-wall related gene expression. The integrated experimental results demonstrated that embryo growth occurred inside imbibed celery fruits in association with endosperm degradation, and that a critical embryo size was required for radicle emergence. The regulation of these processes depends on gene expression leading to gibberellin and indole-3-acetic acid (IAA) production by the embryo and on crosstalk between the fruit compartments. Abscisic acid (ABA) degradation associated with distinct spatiotemporal patterns in ABA sensitivity control embryo growth, endosperm breakdown and radicle emergence. This complex interaction between gibberellins, IAA and ABA metabolism, and changes in the tissue-specific sensitivities to these hormones is distinct from non-MD seeds. We conclude that the embryo growth to reach the critical size and the associated endosperm breakdown inside MD fruits constitute a unique germination programme.

AB - Underdeveloped (small) embryos embedded in abundant endosperm tissue, and thus having morphological (MD) or morphophysiological (MPD) dormancy, are considered to be the ancestral state in seed dormancy evolution. This trait is retained in the Apiaceae family which provides excellent model systems to study the underpinning mechanisms. We investigated Apium graveolens (celery) MD by combined innovative imaging and embryo growth assays with the quantification of hormone metabolism, as well as the analysis of hormone and cell-wall related gene expression. The integrated experimental results demonstrated that embryo growth occurred inside imbibed celery fruits in association with endosperm degradation, and that a critical embryo size was required for radicle emergence. The regulation of these processes depends on gene expression leading to gibberellin and indole-3-acetic acid (IAA) production by the embryo and on crosstalk between the fruit compartments. Abscisic acid (ABA) degradation associated with distinct spatiotemporal patterns in ABA sensitivity control embryo growth, endosperm breakdown and radicle emergence. This complex interaction between gibberellins, IAA and ABA metabolism, and changes in the tissue-specific sensitivities to these hormones is distinct from non-MD seeds. We conclude that the embryo growth to reach the critical size and the associated endosperm breakdown inside MD fruits constitute a unique germination programme.

U2 - 10.1111/tpj.15489

DO - 10.1111/tpj.15489

M3 - Article

JO - Plant Journal

JF - Plant Journal

SN - 0960-7412

ER -