Isolation and characterisation of sub-plastidial fractions from carotenoid rich fruits. Isolation of chromoplast sub-plastidial fractions. / Berry, Harriet; Nogueira, Marilise; Drapal, Margit; Almeida Barros da Silva, Juliana; Perez, Laura; Enfissi, Genny; Fraser, Paul.

Isolation and characterisation of sub-plastidial fractions from carotenoid rich fruits. : Isolation and characterisation of sub-plastidial fractions from carotenoid rich fruits. . ed. / Eleanore Wurtzel. Methods in Enzymology : Elsevier, 2022.

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Abstract

Carotenoid biosynthesis and sequestration in higher plants occurs in the plastid organelle. Among diverse germplasm collections displaying natural variation for carotenoids and outputs from metabolic engineering experiments it has become clear that plastid type and numbers can have important implications on the quantitative composition of carotenoids accumulating. Therefore, it is important to characterise these organelles to fully evaluate the potential of the germplasm to enhance carotenoids and create nutrient dense fruits and vegetables. In this article the procedures used to isolate sub-plastidial structures from carotenoid-rich Solanaceae fruits (tomato and Capsicum) are described.
Original languageEnglish
Title of host publicationIsolation and characterisation of sub-plastidial fractions from carotenoid rich fruits.
Subtitle of host publicationIsolation and characterisation of sub-plastidial fractions from carotenoid rich fruits.
EditorsEleanore Wurtzel
Place of PublicationMethods in Enzymology
PublisherElsevier
ISBN (Electronic)97803233999755
Publication statusE-pub ahead of print - 17 Apr 2022

ID: 45213673