Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease. / Lu-Nguyen, Ngoc; Broadstock, Martin; Yáñez-Muñoz, Rafael J.

In: Methods in Molecular Biology, Vol. 1448, 18.06.2016, p. 175-184.

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Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease. / Lu-Nguyen, Ngoc; Broadstock, Martin; Yáñez-Muñoz, Rafael J.

In: Methods in Molecular Biology, Vol. 1448, 18.06.2016, p. 175-184.

Research output: Contribution to journalArticlepeer-review

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@article{7e7aeea8e25045e3968ead5c92016d7a,
title = "Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson{\textquoteright}s Disease",
abstract = "Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson{\textquoteright}s disease, and (3) postmortem brain processing.",
author = "Ngoc Lu-Nguyen and Martin Broadstock and Y{\'a}{\~n}ez-Mu{\~n}oz, {Rafael J.}",
year = "2016",
month = jun,
day = "18",
doi = "10.1007/978-1-4939-3753-0_13",
language = "English",
volume = "1448",
pages = "175--184",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease

AU - Lu-Nguyen, Ngoc

AU - Broadstock, Martin

AU - Yáñez-Muñoz, Rafael J.

PY - 2016/6/18

Y1 - 2016/6/18

N2 - Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson’s disease, and (3) postmortem brain processing.

AB - Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson’s disease, and (3) postmortem brain processing.

U2 - 10.1007/978-1-4939-3753-0_13

DO - 10.1007/978-1-4939-3753-0_13

M3 - Article

VL - 1448

SP - 175

EP - 184

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -