Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease. / Lu-Nguyen, Ngoc; Broadstock, Martin; Yáñez-Muñoz, Rafael J.

In: Methods in Molecular Biology, Vol. 1448, 18.06.2016, p. 175-184.

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Abstract

Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson’s disease, and (3) postmortem brain processing.
Original languageEnglish
Pages (from-to)175-184
Number of pages10
JournalMethods in Molecular Biology
Volume1448
Early online date18 Jun 2016
DOIs
Publication statusE-pub ahead of print - 18 Jun 2016

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