In vivo 1H NMR microimaging during seed imbibition, germination and early growth: Methods in Molecular Biology: Seed Dormancy

V. Terskikh, K. Müller, A. R. Kermode, G. Leubner-Metzger

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Magnetic resonance imaging (MRI) is a superior noninvasive diagnostic tool widely used in clinical medicine, with more than 60 million MRI tests performed each year worldwide. More specialized high-resolution MRI systems capable of a resolution that is 100-1,000 times higher than standard MRI instruments are used primarily in materials science, but are used with increasing frequency in plant physiology. We have shown that high-resolution (1)H-nuclear magnetic resonance (NMR) microimaging can provide a wealth of information about the internal anatomy of plant seeds as small as 1 mm or even smaller. This chapter covers the methods associated with these imaging techniques in detail. We also discuss the application of (1)H-NMR microimaging to study in vivo seed imbibition, germination, and early seedling growth.
Original languageEnglish
Title of host publicationMethods in Molecular Biology: Seed Dormancy
EditorsA. R. Kermode
PublisherSpringer
Publication statusPublished - 2010

Cite this