Impact of pH on the viability and morphology of Blastocystis isolates. / Farah Haziqah, M.T; Chandrawathani, P; Benacer, Douadi; Suresh, K; Wilson, J.J; Mohd Khalid, M.K.N; Rajamanikam, A; Lewis, John; Mohd Zain, SN.

In: Tropical Biomedicine, Vol. 35, No. 2, 06.2018, p. 501-510.

Research output: Contribution to journalArticlepeer-review

Published

Standard

Impact of pH on the viability and morphology of Blastocystis isolates. / Farah Haziqah, M.T; Chandrawathani, P; Benacer, Douadi; Suresh, K; Wilson, J.J; Mohd Khalid, M.K.N; Rajamanikam, A; Lewis, John; Mohd Zain, SN.

In: Tropical Biomedicine, Vol. 35, No. 2, 06.2018, p. 501-510.

Research output: Contribution to journalArticlepeer-review

Harvard

Farah Haziqah, MT, Chandrawathani, P, Benacer, D, Suresh, K, Wilson, JJ, Mohd Khalid, MKN, Rajamanikam, A, Lewis, J & Mohd Zain, SN 2018, 'Impact of pH on the viability and morphology of Blastocystis isolates', Tropical Biomedicine, vol. 35, no. 2, pp. 501-510. <http://msptm.org/files/Vol35No2/501-510-Mohd-Zain-SN.pdf>

APA

Farah Haziqah, M. T., Chandrawathani, P., Benacer, D., Suresh, K., Wilson, J. J., Mohd Khalid, M. K. N., Rajamanikam, A., Lewis, J., & Mohd Zain, SN. (2018). Impact of pH on the viability and morphology of Blastocystis isolates. Tropical Biomedicine, 35(2), 501-510. http://msptm.org/files/Vol35No2/501-510-Mohd-Zain-SN.pdf

Vancouver

Farah Haziqah MT, Chandrawathani P, Benacer D, Suresh K, Wilson JJ, Mohd Khalid MKN et al. Impact of pH on the viability and morphology of Blastocystis isolates. Tropical Biomedicine. 2018 Jun;35(2):501-510.

Author

Farah Haziqah, M.T ; Chandrawathani, P ; Benacer, Douadi ; Suresh, K ; Wilson, J.J ; Mohd Khalid, M.K.N ; Rajamanikam, A ; Lewis, John ; Mohd Zain, SN. / Impact of pH on the viability and morphology of Blastocystis isolates. In: Tropical Biomedicine. 2018 ; Vol. 35, No. 2. pp. 501-510.

BibTeX

@article{1990fe94b0f74fc1ab13ba72234e1ad6,
title = "Impact of pH on the viability and morphology of Blastocystis isolates",
abstract = "Blastocystis sp. is ubiquitous in avian, mammalian and human hosts and propagates in either neutral or slightly alkaline conditions within the host{\textquoteright}s gastro-intestinal tract. Of the few previous studies on this enteric protozoan parasite in feline and canine hosts, prevalence values have been shown to range between 0 to 70.8%. In view of the close association between humans, and canine and feline hosts as companion animals, faecal samples of 180 Felis catus and 82 Canis lupus, collected from Penang and Kuala Lumpur, Malaysia, were initially screened by in vitro cultivation followed by molecular characterization. No positive isolates were identified in culture but in 12 feline samples DNA barcoding detected a zoonotic subtype Blastocystis ST1 for the first time. Consequently, avian and human isolates, which had previously been successfully cultured, were used to investigate the impact of pH on the viability and morphology of Blastocystis sp. The use of Trypan blue showed that the number of viable cells increased when exposed to pH 4 and a significant increase in viability occurred in pH values of 5 to 7. Development of Blastocystis cells in both isolates was suppressed in media less than pH 5 followed by the disappearance of viable cells from avian isolates in more acidic media below pH 4. Morphologically at pH 4 cells from avian isolates were less rounded, and with wrinkled / shrunken surfaces, than the more normal rounded cells from human isolates. On the other hand, at values below pH 3, no viable cells in human isolates were visible. The present findings therefore confirm that gastro-intestinal pH is an important determinant of Blastocystis viability and consequently influences the epidemiology of infection within avian, mammalian and human hosts.",
author = "{Farah Haziqah}, M.T and P Chandrawathani and Douadi Benacer and K Suresh and J.J Wilson and {Mohd Khalid}, M.K.N and A Rajamanikam and John Lewis and {Mohd Zain}, SN",
year = "2018",
month = jun,
language = "English",
volume = "35",
pages = "501--510",
journal = "Tropical Biomedicine",
issn = "0127-5720",
publisher = "Malaysian Society of Parasitology and Tropical Medicine",
number = "2",

}

RIS

TY - JOUR

T1 - Impact of pH on the viability and morphology of Blastocystis isolates

AU - Farah Haziqah, M.T

AU - Chandrawathani, P

AU - Benacer, Douadi

AU - Suresh, K

AU - Wilson, J.J

AU - Mohd Khalid, M.K.N

AU - Rajamanikam, A

AU - Lewis, John

AU - Mohd Zain, SN

PY - 2018/6

Y1 - 2018/6

N2 - Blastocystis sp. is ubiquitous in avian, mammalian and human hosts and propagates in either neutral or slightly alkaline conditions within the host’s gastro-intestinal tract. Of the few previous studies on this enteric protozoan parasite in feline and canine hosts, prevalence values have been shown to range between 0 to 70.8%. In view of the close association between humans, and canine and feline hosts as companion animals, faecal samples of 180 Felis catus and 82 Canis lupus, collected from Penang and Kuala Lumpur, Malaysia, were initially screened by in vitro cultivation followed by molecular characterization. No positive isolates were identified in culture but in 12 feline samples DNA barcoding detected a zoonotic subtype Blastocystis ST1 for the first time. Consequently, avian and human isolates, which had previously been successfully cultured, were used to investigate the impact of pH on the viability and morphology of Blastocystis sp. The use of Trypan blue showed that the number of viable cells increased when exposed to pH 4 and a significant increase in viability occurred in pH values of 5 to 7. Development of Blastocystis cells in both isolates was suppressed in media less than pH 5 followed by the disappearance of viable cells from avian isolates in more acidic media below pH 4. Morphologically at pH 4 cells from avian isolates were less rounded, and with wrinkled / shrunken surfaces, than the more normal rounded cells from human isolates. On the other hand, at values below pH 3, no viable cells in human isolates were visible. The present findings therefore confirm that gastro-intestinal pH is an important determinant of Blastocystis viability and consequently influences the epidemiology of infection within avian, mammalian and human hosts.

AB - Blastocystis sp. is ubiquitous in avian, mammalian and human hosts and propagates in either neutral or slightly alkaline conditions within the host’s gastro-intestinal tract. Of the few previous studies on this enteric protozoan parasite in feline and canine hosts, prevalence values have been shown to range between 0 to 70.8%. In view of the close association between humans, and canine and feline hosts as companion animals, faecal samples of 180 Felis catus and 82 Canis lupus, collected from Penang and Kuala Lumpur, Malaysia, were initially screened by in vitro cultivation followed by molecular characterization. No positive isolates were identified in culture but in 12 feline samples DNA barcoding detected a zoonotic subtype Blastocystis ST1 for the first time. Consequently, avian and human isolates, which had previously been successfully cultured, were used to investigate the impact of pH on the viability and morphology of Blastocystis sp. The use of Trypan blue showed that the number of viable cells increased when exposed to pH 4 and a significant increase in viability occurred in pH values of 5 to 7. Development of Blastocystis cells in both isolates was suppressed in media less than pH 5 followed by the disappearance of viable cells from avian isolates in more acidic media below pH 4. Morphologically at pH 4 cells from avian isolates were less rounded, and with wrinkled / shrunken surfaces, than the more normal rounded cells from human isolates. On the other hand, at values below pH 3, no viable cells in human isolates were visible. The present findings therefore confirm that gastro-intestinal pH is an important determinant of Blastocystis viability and consequently influences the epidemiology of infection within avian, mammalian and human hosts.

M3 - Article

VL - 35

SP - 501

EP - 510

JO - Tropical Biomedicine

JF - Tropical Biomedicine

SN - 0127-5720

IS - 2

ER -