Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator. / Koch, Hauke; Welcome, Vita; Kendal-Smith, Amy; Thursfield, Lucy; Farrell, Iain W; Langat, Moses K; Brown, Mark J F; Stevenson, Philip C.

In: Philosophical Transactions of the Royal Society of London B: Biological Sciences, 02.05.2022.

Research output: Contribution to journalArticlepeer-review

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Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator. / Koch, Hauke; Welcome, Vita; Kendal-Smith, Amy; Thursfield, Lucy; Farrell, Iain W; Langat, Moses K; Brown, Mark J F; Stevenson, Philip C.

In: Philosophical Transactions of the Royal Society of London B: Biological Sciences, 02.05.2022.

Research output: Contribution to journalArticlepeer-review

Harvard

Koch, H, Welcome, V, Kendal-Smith, A, Thursfield, L, Farrell, IW, Langat, MK, Brown, MJF & Stevenson, PC 2022, 'Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator', Philosophical Transactions of the Royal Society of London B: Biological Sciences. https://doi.org/10.1098/rstb.2021.0162

APA

Koch, H., Welcome, V., Kendal-Smith, A., Thursfield, L., Farrell, I. W., Langat, M. K., Brown, M. J. F., & Stevenson, P. C. (2022). Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator. Philosophical Transactions of the Royal Society of London B: Biological Sciences. https://doi.org/10.1098/rstb.2021.0162

Vancouver

Koch H, Welcome V, Kendal-Smith A, Thursfield L, Farrell IW, Langat MK et al. Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator. Philosophical Transactions of the Royal Society of London B: Biological Sciences. 2022 May 2. https://doi.org/10.1098/rstb.2021.0162

Author

Koch, Hauke ; Welcome, Vita ; Kendal-Smith, Amy ; Thursfield, Lucy ; Farrell, Iain W ; Langat, Moses K ; Brown, Mark J F ; Stevenson, Philip C. / Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator. In: Philosophical Transactions of the Royal Society of London B: Biological Sciences. 2022.

BibTeX

@article{767a2e7cf35d4f858b6e425ed488b629,
title = "Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator",
abstract = "Antimicrobial nectar secondary metabolites can support pollinator health by preventing or reducing parasite infections. To better understand the outcome of nectar metabolite-parasite interactions in pollinators, we determined whether the antiparasitic activity was altered through chemical modification by the host or resident microbiome during gut passage. We investigated this interaction with linden (Tilia spp.) and strawberry tree (Arbutus unedo) nectar compounds. Unedone from A. unedo nectar inhibited the common bumblebee gut parasite Crithidia bombi in vitro and in Bombus terrestris gynes. A compound in Tilia nectar, 1-[4-(1-hydroxy-1-methylethyl)-1,3-cyclohexadiene-1-carboxylate]-6-O-β-D-glucopyranosyl-β-D-glucopyranose (tiliaside) showed no inhibition in vitro at naturally occurring concentrations, but reduced C. bombi infections of B. terrestris workers. Independent of microbiome status, tiliaside was deglycosylated during gut passage, thereby increasing its antiparasitic activity in the hindgut, the site of C. bombi infections. Conversely, unedone was first glycosylated in the midgut without influence of the microbiome to unedone-8-O-β-D-glucoside, rendering it inactive against C. bombi, but subsequently deglycosylated by the microbiome in the hindgut, restoring its activity. We therefore show that conversion of nectar metabolites by either the host or the microbiome modulates antiparasitic activity.",
author = "Hauke Koch and Vita Welcome and Amy Kendal-Smith and Lucy Thursfield and Farrell, {Iain W} and Langat, {Moses K} and Brown, {Mark J F} and Stevenson, {Philip C.}",
year = "2022",
month = may,
day = "2",
doi = "10.1098/rstb.2021.0162",
language = "English",
journal = "Philosophical Transactions of the Royal Society of London B: Biological Sciences",
issn = "0962-8436",
publisher = "Royal Society of London",

}

RIS

TY - JOUR

T1 - Host and gut microbiome modulate the antiparasitic activity of nectar metabolites in a bumblebee pollinator

AU - Koch, Hauke

AU - Welcome, Vita

AU - Kendal-Smith, Amy

AU - Thursfield, Lucy

AU - Farrell, Iain W

AU - Langat, Moses K

AU - Brown, Mark J F

AU - Stevenson, Philip C.

PY - 2022/5/2

Y1 - 2022/5/2

N2 - Antimicrobial nectar secondary metabolites can support pollinator health by preventing or reducing parasite infections. To better understand the outcome of nectar metabolite-parasite interactions in pollinators, we determined whether the antiparasitic activity was altered through chemical modification by the host or resident microbiome during gut passage. We investigated this interaction with linden (Tilia spp.) and strawberry tree (Arbutus unedo) nectar compounds. Unedone from A. unedo nectar inhibited the common bumblebee gut parasite Crithidia bombi in vitro and in Bombus terrestris gynes. A compound in Tilia nectar, 1-[4-(1-hydroxy-1-methylethyl)-1,3-cyclohexadiene-1-carboxylate]-6-O-β-D-glucopyranosyl-β-D-glucopyranose (tiliaside) showed no inhibition in vitro at naturally occurring concentrations, but reduced C. bombi infections of B. terrestris workers. Independent of microbiome status, tiliaside was deglycosylated during gut passage, thereby increasing its antiparasitic activity in the hindgut, the site of C. bombi infections. Conversely, unedone was first glycosylated in the midgut without influence of the microbiome to unedone-8-O-β-D-glucoside, rendering it inactive against C. bombi, but subsequently deglycosylated by the microbiome in the hindgut, restoring its activity. We therefore show that conversion of nectar metabolites by either the host or the microbiome modulates antiparasitic activity.

AB - Antimicrobial nectar secondary metabolites can support pollinator health by preventing or reducing parasite infections. To better understand the outcome of nectar metabolite-parasite interactions in pollinators, we determined whether the antiparasitic activity was altered through chemical modification by the host or resident microbiome during gut passage. We investigated this interaction with linden (Tilia spp.) and strawberry tree (Arbutus unedo) nectar compounds. Unedone from A. unedo nectar inhibited the common bumblebee gut parasite Crithidia bombi in vitro and in Bombus terrestris gynes. A compound in Tilia nectar, 1-[4-(1-hydroxy-1-methylethyl)-1,3-cyclohexadiene-1-carboxylate]-6-O-β-D-glucopyranosyl-β-D-glucopyranose (tiliaside) showed no inhibition in vitro at naturally occurring concentrations, but reduced C. bombi infections of B. terrestris workers. Independent of microbiome status, tiliaside was deglycosylated during gut passage, thereby increasing its antiparasitic activity in the hindgut, the site of C. bombi infections. Conversely, unedone was first glycosylated in the midgut without influence of the microbiome to unedone-8-O-β-D-glucoside, rendering it inactive against C. bombi, but subsequently deglycosylated by the microbiome in the hindgut, restoring its activity. We therefore show that conversion of nectar metabolites by either the host or the microbiome modulates antiparasitic activity.

U2 - 10.1098/rstb.2021.0162

DO - 10.1098/rstb.2021.0162

M3 - Article

JO - Philosophical Transactions of the Royal Society of London B: Biological Sciences

JF - Philosophical Transactions of the Royal Society of London B: Biological Sciences

SN - 0962-8436

ER -