Extending our tools and resources in the non-conventional industrial yeast Xanthophyllomyces dendrorhous through the application of metabolite profiling methodologies. / Alcalde Rodriguez, Eugenio; Fraser, Paul.

In: Metabolomics, 21.12.2017.

Research output: Contribution to journalArticle

Forthcoming

Abstract

Xanthophyllomyces dendrorhous is a non-conventional industrial yeast. Among yeasts it has the unique ability to produce carotenoids in particular astaxanthin. The Xanthophyllomyces genome has now been sequenced and annotated.
Objective.In order to further the “omic” resources available in this organism metabolite profiling workflows have been developed and described in this present article. To achieve an efficient metabolite profiling system, quenching, extraction and associated GC-MS and UPLC analytics have been optimised.
Method.Four quenching methods and five extraction methods were tested and validated by analysing the metabolite changes of Xanthophyllomyces in a liquid shaking culture at lag, exponential and early and late stationary phases.
Results.A customised Automated Mass Spectral Deconvolution and Identification System (AMDIS) library has been created for Xanthophyllomyces, over 400 compounds are present in the library of which 70 are detected and quantified routinely in polar and non-polar derived extracts. A preliminary biochemical network has been constructed. Over a standardised laboratory growth cycle, with lag, exponential and stationary phases assigned on the basis of growth characteristic growth parameters. The metabolite profiling procedures have been used to determine the basal levels and changes in metabolites. Correlation analysis has illustrated that the astaxanthin correlate positively with different sectors of intermediary metabolism (e.g. the TCA cycle intermediates and amino acid formation), “short” saturated fatty acids and β-carotene, while other metabolites are reduced in response to astaxanthin production. These sectors of intermediary offer potential future targets for the manipulation of metabolism in this organism for the production of valuable compounds.
Discussion.In summary a robust metabolite profiling system for Xanthophyllomyces is in place to further our understanding and potential exploitation of this underutilised industrial yeast.
Original languageEnglish
JournalMetabolomics
StateAccepted/In press - 21 Dec 2017

ID: 28961645