Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. / Nogueira, Marilise; Enfissi, Eugenia; Martínez Valenzuela, Maria; Menard, Guillaume; Driller, Richard L; Eastmond, Peter J; Schuch, Wolfgang; Sandmann, Gerhard; Fraser, Paul.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 41, 10.10.2017, p. 10876–10881.

Research output: Contribution to journalArticlepeer-review

Published

Standard

Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. / Nogueira, Marilise; Enfissi, Eugenia; Martínez Valenzuela, Maria; Menard, Guillaume; Driller, Richard L; Eastmond, Peter J; Schuch, Wolfgang; Sandmann, Gerhard; Fraser, Paul.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, No. 41, 10.10.2017, p. 10876–10881.

Research output: Contribution to journalArticlepeer-review

Harvard

Nogueira, M, Enfissi, E, Martínez Valenzuela, M, Menard, G, Driller, RL, Eastmond, PJ, Schuch, W, Sandmann, G & Fraser, P 2017, 'Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed', Proceedings of the National Academy of Sciences of the United States of America, vol. 114, no. 41, pp. 10876–10881. https://doi.org/10.1073/pnas.1708349114

APA

Nogueira, M., Enfissi, E., Martínez Valenzuela, M., Menard, G., Driller, R. L., Eastmond, P. J., Schuch, W., Sandmann, G., & Fraser, P. (2017). Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. Proceedings of the National Academy of Sciences of the United States of America, 114(41), 10876–10881. https://doi.org/10.1073/pnas.1708349114

Vancouver

Nogueira M, Enfissi E, Martínez Valenzuela M, Menard G, Driller RL, Eastmond PJ et al. Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. Proceedings of the National Academy of Sciences of the United States of America. 2017 Oct 10;114(41):10876–10881. https://doi.org/10.1073/pnas.1708349114

Author

Nogueira, Marilise ; Enfissi, Eugenia ; Martínez Valenzuela, Maria ; Menard, Guillaume ; Driller, Richard L ; Eastmond, Peter J ; Schuch, Wolfgang ; Sandmann, Gerhard ; Fraser, Paul. / Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed. In: Proceedings of the National Academy of Sciences of the United States of America. 2017 ; Vol. 114, No. 41. pp. 10876–10881.

BibTeX

@article{e0f2211103734ddab4b08ae104899bd0,
title = "Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed",
abstract = "Ketocarotenoids are high-value pigments used commercially across multiple industrial sectors as colorants and supplements. Chemical synthesis using petrochemical-derived precursors remains the production method of choice. Aquaculture is an example where ketocarotenoid supplementation of feed is necessary to achieve product viability. The biosynthesis of ketocarotenoids, such as canthaxanthin, phoenicoxanthin, or astaxanthin in plants is rare. In the present study, complex engineering of the carotenoid pathway has been performed to produce high-value ketocarotenoids in tomato fruit (3.0 mg/g dry weight). The strategy adopted involved pathway extension beyond β-carotene through the expression of the β-carotene hydroxylase (CrtZ) and oxyxgenase (CrtW) from Brevundimonas sp. in tomato fruit, followed by β-carotene enhancement through the introgression of a lycopene β-cyclase (β-Cyc) allele from a Solanum galapagense background. Detailed biochemical analysis, carried out using chromatographic, UV/VIS, and MS approaches, identified the predominant carotenoid as fatty acid (C14:0 and C16:0) esters of phoenicoxanthin, present in the S stereoisomer configuration. Under a field-like environment with low resource input, scalability was shown with the potential to deliver 23 kg of ketocarotenoid/hectare. To illustrate the potential of this “generally recognized as safe” material with minimal, low-energy bioprocessing, two independent aquaculture trials were performed. The plant-based feeds developed were more efficient than the synthetic feed to color trout flesh (up to twofold increase in the retention of the main ketocarotenoids in the fish fillets). This achievement has the potential to create a new paradigm in the renewable production of economically competitive feed additives for the aquaculture industry and beyond.",
keywords = "Ketocarotenoids for aquaculture feed",
author = "Marilise Nogueira and Eugenia Enfissi and {Mart{\'i}nez Valenzuela}, Maria and Guillaume Menard and Driller, {Richard L} and Eastmond, {Peter J} and Wolfgang Schuch and Gerhard Sandmann and Paul Fraser",
year = "2017",
month = oct,
day = "10",
doi = "10.1073/pnas.1708349114",
language = "English",
volume = "114",
pages = "10876–10881",
journal = " Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "41",

}

RIS

TY - JOUR

T1 - Engineering of tomato for the sustainable production of ketocarotenoids and its evaluation in aquaculture feed

AU - Nogueira, Marilise

AU - Enfissi, Eugenia

AU - Martínez Valenzuela, Maria

AU - Menard, Guillaume

AU - Driller, Richard L

AU - Eastmond, Peter J

AU - Schuch, Wolfgang

AU - Sandmann, Gerhard

AU - Fraser, Paul

PY - 2017/10/10

Y1 - 2017/10/10

N2 - Ketocarotenoids are high-value pigments used commercially across multiple industrial sectors as colorants and supplements. Chemical synthesis using petrochemical-derived precursors remains the production method of choice. Aquaculture is an example where ketocarotenoid supplementation of feed is necessary to achieve product viability. The biosynthesis of ketocarotenoids, such as canthaxanthin, phoenicoxanthin, or astaxanthin in plants is rare. In the present study, complex engineering of the carotenoid pathway has been performed to produce high-value ketocarotenoids in tomato fruit (3.0 mg/g dry weight). The strategy adopted involved pathway extension beyond β-carotene through the expression of the β-carotene hydroxylase (CrtZ) and oxyxgenase (CrtW) from Brevundimonas sp. in tomato fruit, followed by β-carotene enhancement through the introgression of a lycopene β-cyclase (β-Cyc) allele from a Solanum galapagense background. Detailed biochemical analysis, carried out using chromatographic, UV/VIS, and MS approaches, identified the predominant carotenoid as fatty acid (C14:0 and C16:0) esters of phoenicoxanthin, present in the S stereoisomer configuration. Under a field-like environment with low resource input, scalability was shown with the potential to deliver 23 kg of ketocarotenoid/hectare. To illustrate the potential of this “generally recognized as safe” material with minimal, low-energy bioprocessing, two independent aquaculture trials were performed. The plant-based feeds developed were more efficient than the synthetic feed to color trout flesh (up to twofold increase in the retention of the main ketocarotenoids in the fish fillets). This achievement has the potential to create a new paradigm in the renewable production of economically competitive feed additives for the aquaculture industry and beyond.

AB - Ketocarotenoids are high-value pigments used commercially across multiple industrial sectors as colorants and supplements. Chemical synthesis using petrochemical-derived precursors remains the production method of choice. Aquaculture is an example where ketocarotenoid supplementation of feed is necessary to achieve product viability. The biosynthesis of ketocarotenoids, such as canthaxanthin, phoenicoxanthin, or astaxanthin in plants is rare. In the present study, complex engineering of the carotenoid pathway has been performed to produce high-value ketocarotenoids in tomato fruit (3.0 mg/g dry weight). The strategy adopted involved pathway extension beyond β-carotene through the expression of the β-carotene hydroxylase (CrtZ) and oxyxgenase (CrtW) from Brevundimonas sp. in tomato fruit, followed by β-carotene enhancement through the introgression of a lycopene β-cyclase (β-Cyc) allele from a Solanum galapagense background. Detailed biochemical analysis, carried out using chromatographic, UV/VIS, and MS approaches, identified the predominant carotenoid as fatty acid (C14:0 and C16:0) esters of phoenicoxanthin, present in the S stereoisomer configuration. Under a field-like environment with low resource input, scalability was shown with the potential to deliver 23 kg of ketocarotenoid/hectare. To illustrate the potential of this “generally recognized as safe” material with minimal, low-energy bioprocessing, two independent aquaculture trials were performed. The plant-based feeds developed were more efficient than the synthetic feed to color trout flesh (up to twofold increase in the retention of the main ketocarotenoids in the fish fillets). This achievement has the potential to create a new paradigm in the renewable production of economically competitive feed additives for the aquaculture industry and beyond.

KW - Ketocarotenoids for aquaculture feed

U2 - 10.1073/pnas.1708349114

DO - 10.1073/pnas.1708349114

M3 - Article

VL - 114

SP - 10876

EP - 10881

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 41

ER -