Directed and Modular Protein Immobilization on Gold and Silver Nanoparticles. / Saccardo, Angela; Ma, Wenwei; Soloviev, Mikhail; Ferrari, Enrico.

In: Methods in Molecular Biology, Vol. 2118, 10.03.2020, p. 227-234.

Research output: Contribution to journalArticle

E-pub ahead of print

Abstract

Conjugation of proteins to gold nanoparticles (AuNP), silver nanoparticles (AgNP), or other metal nanoparticles (NPs) can often be achieved using passive adsorption. Although such an approach is simple and effective, there is usually no control over the orientation of the protein and denaturation due to close contact with the metal surface. The method described here makes use of adapter proteins which have the ability to adsorb to the NP surface in an oriented and stable way and at the same time enable straightforward attachment to other proteins of interest.

Original languageEnglish
Pages (from-to)227-234
Number of pages8
JournalMethods in Molecular Biology
Volume2118
DOIs
Publication statusE-pub ahead of print - 10 Mar 2020
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

ID: 37536270