Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02. / Cherdchukeattisak, Peelada; Fraser, Paul; Purton, Saul; Wannathong Brocklehurst, Thanyanan.

In: Open biology, Vol. 7, No. 1, 17, 12.02.2018, p. 1-14.

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Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02. / Cherdchukeattisak, Peelada; Fraser, Paul; Purton, Saul; Wannathong Brocklehurst, Thanyanan.

In: Open biology, Vol. 7, No. 1, 17, 12.02.2018, p. 1-14.

Research output: Contribution to journalArticlepeer-review

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Cherdchukeattisak, Peelada ; Fraser, Paul ; Purton, Saul ; Wannathong Brocklehurst, Thanyanan. / Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02. In: Open biology. 2018 ; Vol. 7, No. 1. pp. 1-14.

BibTeX

@article{c40fab17d61e4caab65305f3b052c539,
title = "Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02",
abstract = "The sarcinoid alga PY02 is a newly isolated soil alga native to western Thailand. In this study PY02 is described, the carotenoid profile of the green and red forms of the algal cells are compared, and the effect of nitrogen reduction and media volume on ketocarotenoid production are reported. Partial sequences of the genes from elongation factor Tu (tufA) and 18S rRNA genes reveal that the alga is from the Chlorosarcinopsis genus. Growth studies demonstrated that Chlorosarcinopsis PY02 is capable of photoautotrophic, heterotrophic and mixotrophic growth. A gradual change in colony colour from green to red was observed over a period of four weeks under mixotrophic conditions. Pigment analysis of lyophilized red cells using ultrahigh performance liquid chromatography (UPLC) with Photo Diode Array Detection (PDA), showed for the first time that an alga from the genus Chlorosarcinopsis is capable of producing ketocarotenoids such as adonixanthin and 3-OH-echinenone, with canthaxanthin as the dominant pigment. Interestingly, a reduction of nitrogen in the medium exerts a positive effect on the rate of colour change from one month to less than seven days. Enhancements of the canthaxanthin content from 520 to 1504 or 1427 µg·g DW−1 were detected under 50% and 10% nitrogen content, respectively. An increase of 16% in biomass production of PY02 was unexpectedly detected from a 50% nitrogen reduction under mixotrophic culture. Notably, in liquid mixotrophic media with volumes of 15, 30 and 60 mL, the lowest volume produced a significantly higher biomass and canthaxanthin content.",
author = "Peelada Cherdchukeattisak and Paul Fraser and Saul Purton and {Wannathong Brocklehurst}, Thanyanan",
year = "2018",
month = feb,
day = "12",
doi = "10.3390/biology7010017",
language = "English",
volume = "7",
pages = "1--14",
journal = "Open biology",
issn = "2046-2441",
publisher = "Royal Society Publishing",
number = "1",

}

RIS

TY - JOUR

T1 - Detection and Enhancement of Ketocarotenoid Accumulation in the Newly Isolated Sarcinoid Green Microalga Chlorosarcinopsis PY02

AU - Cherdchukeattisak, Peelada

AU - Fraser, Paul

AU - Purton, Saul

AU - Wannathong Brocklehurst, Thanyanan

PY - 2018/2/12

Y1 - 2018/2/12

N2 - The sarcinoid alga PY02 is a newly isolated soil alga native to western Thailand. In this study PY02 is described, the carotenoid profile of the green and red forms of the algal cells are compared, and the effect of nitrogen reduction and media volume on ketocarotenoid production are reported. Partial sequences of the genes from elongation factor Tu (tufA) and 18S rRNA genes reveal that the alga is from the Chlorosarcinopsis genus. Growth studies demonstrated that Chlorosarcinopsis PY02 is capable of photoautotrophic, heterotrophic and mixotrophic growth. A gradual change in colony colour from green to red was observed over a period of four weeks under mixotrophic conditions. Pigment analysis of lyophilized red cells using ultrahigh performance liquid chromatography (UPLC) with Photo Diode Array Detection (PDA), showed for the first time that an alga from the genus Chlorosarcinopsis is capable of producing ketocarotenoids such as adonixanthin and 3-OH-echinenone, with canthaxanthin as the dominant pigment. Interestingly, a reduction of nitrogen in the medium exerts a positive effect on the rate of colour change from one month to less than seven days. Enhancements of the canthaxanthin content from 520 to 1504 or 1427 µg·g DW−1 were detected under 50% and 10% nitrogen content, respectively. An increase of 16% in biomass production of PY02 was unexpectedly detected from a 50% nitrogen reduction under mixotrophic culture. Notably, in liquid mixotrophic media with volumes of 15, 30 and 60 mL, the lowest volume produced a significantly higher biomass and canthaxanthin content.

AB - The sarcinoid alga PY02 is a newly isolated soil alga native to western Thailand. In this study PY02 is described, the carotenoid profile of the green and red forms of the algal cells are compared, and the effect of nitrogen reduction and media volume on ketocarotenoid production are reported. Partial sequences of the genes from elongation factor Tu (tufA) and 18S rRNA genes reveal that the alga is from the Chlorosarcinopsis genus. Growth studies demonstrated that Chlorosarcinopsis PY02 is capable of photoautotrophic, heterotrophic and mixotrophic growth. A gradual change in colony colour from green to red was observed over a period of four weeks under mixotrophic conditions. Pigment analysis of lyophilized red cells using ultrahigh performance liquid chromatography (UPLC) with Photo Diode Array Detection (PDA), showed for the first time that an alga from the genus Chlorosarcinopsis is capable of producing ketocarotenoids such as adonixanthin and 3-OH-echinenone, with canthaxanthin as the dominant pigment. Interestingly, a reduction of nitrogen in the medium exerts a positive effect on the rate of colour change from one month to less than seven days. Enhancements of the canthaxanthin content from 520 to 1504 or 1427 µg·g DW−1 were detected under 50% and 10% nitrogen content, respectively. An increase of 16% in biomass production of PY02 was unexpectedly detected from a 50% nitrogen reduction under mixotrophic culture. Notably, in liquid mixotrophic media with volumes of 15, 30 and 60 mL, the lowest volume produced a significantly higher biomass and canthaxanthin content.

U2 - 10.3390/biology7010017

DO - 10.3390/biology7010017

M3 - Article

VL - 7

SP - 1

EP - 14

JO - Open biology

JF - Open biology

SN - 2046-2441

IS - 1

M1 - 17

ER -