Delta-Like 4 Activates Notch 3 to Regulate Self-Renewal in Skeletal Muscle Stem Cells. / Low, Siew Hui; Barnes, Josephine; Zammit, Peter ; Beauchamp, Jonathan.

In: Stem Cells, Vol. 36, No. 3, 03.2018, p. 458-466.

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Delta-Like 4 Activates Notch 3 to Regulate Self-Renewal in Skeletal Muscle Stem Cells. / Low, Siew Hui; Barnes, Josephine; Zammit, Peter ; Beauchamp, Jonathan.

In: Stem Cells, Vol. 36, No. 3, 03.2018, p. 458-466.

Research output: Contribution to journalArticlepeer-review

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Low, Siew Hui ; Barnes, Josephine ; Zammit, Peter ; Beauchamp, Jonathan. / Delta-Like 4 Activates Notch 3 to Regulate Self-Renewal in Skeletal Muscle Stem Cells. In: Stem Cells. 2018 ; Vol. 36, No. 3. pp. 458-466.

BibTeX

@article{671e97b125fc45dba09ebaf0876f9a65,
title = "Delta-Like 4 Activates Notch 3 to Regulate Self-Renewal in Skeletal Muscle Stem Cells",
abstract = "Notch signaling is essential to maintain skeletal muscle stem cells in quiescence. However, the precise roles of different Notch receptors are incompletely defined. Here, we demonstrate a role for Notch3 (N3) in the self-renewal of muscle stem cells. We found that N3 is active in quiescent C2C12 reserve cells (RCs), and N3 over-expression and knockdown studies in C2C12 and primary satellite cells reveal a role in self-renewal. The Notch ligand Delta-like 4 (Dll4) is expressed by newly formed myotubes and interaction with this ligand is sufficient to maintain N3 activity in quiescent C2C12 RCs to prevent activation and progression into the cell cycle. Thus, our data suggest a model whereby during regeneration, expression of Dll4 by nascent muscle fibers triggers N3 signaling in associated muscle stem cells to recruit them to quiescence, thereby renewing the stem cell pool. ",
author = "Low, {Siew Hui} and Josephine Barnes and Peter Zammit and Jonathan Beauchamp",
year = "2018",
month = mar,
doi = "10.1002/stem.2757",
language = "English",
volume = "36",
pages = "458--466",
journal = "Stem Cells",
issn = "1549-4918",
publisher = "Wiley",
number = "3",

}

RIS

TY - JOUR

T1 - Delta-Like 4 Activates Notch 3 to Regulate Self-Renewal in Skeletal Muscle Stem Cells

AU - Low, Siew Hui

AU - Barnes, Josephine

AU - Zammit, Peter

AU - Beauchamp, Jonathan

PY - 2018/3

Y1 - 2018/3

N2 - Notch signaling is essential to maintain skeletal muscle stem cells in quiescence. However, the precise roles of different Notch receptors are incompletely defined. Here, we demonstrate a role for Notch3 (N3) in the self-renewal of muscle stem cells. We found that N3 is active in quiescent C2C12 reserve cells (RCs), and N3 over-expression and knockdown studies in C2C12 and primary satellite cells reveal a role in self-renewal. The Notch ligand Delta-like 4 (Dll4) is expressed by newly formed myotubes and interaction with this ligand is sufficient to maintain N3 activity in quiescent C2C12 RCs to prevent activation and progression into the cell cycle. Thus, our data suggest a model whereby during regeneration, expression of Dll4 by nascent muscle fibers triggers N3 signaling in associated muscle stem cells to recruit them to quiescence, thereby renewing the stem cell pool.

AB - Notch signaling is essential to maintain skeletal muscle stem cells in quiescence. However, the precise roles of different Notch receptors are incompletely defined. Here, we demonstrate a role for Notch3 (N3) in the self-renewal of muscle stem cells. We found that N3 is active in quiescent C2C12 reserve cells (RCs), and N3 over-expression and knockdown studies in C2C12 and primary satellite cells reveal a role in self-renewal. The Notch ligand Delta-like 4 (Dll4) is expressed by newly formed myotubes and interaction with this ligand is sufficient to maintain N3 activity in quiescent C2C12 RCs to prevent activation and progression into the cell cycle. Thus, our data suggest a model whereby during regeneration, expression of Dll4 by nascent muscle fibers triggers N3 signaling in associated muscle stem cells to recruit them to quiescence, thereby renewing the stem cell pool.

U2 - 10.1002/stem.2757

DO - 10.1002/stem.2757

M3 - Article

VL - 36

SP - 458

EP - 466

JO - Stem Cells

JF - Stem Cells

SN - 1549-4918

IS - 3

ER -