DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. / Graeber, Kai; Linkies, Ada; Steinbrecher, Tina; Mummenhoff, Klaus; Tarkowska, Danuse; Tureckova, Veronika; Ignatz, Michael; Sperber, Katja; Voegele, Antje; de Jong, Hans; Urbanova, Terezie; Strnad, Miroslav; Leubner-Metzger, Gerhard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 34, 26.08.2014, p. E3571-E3580.

Research output: Contribution to journalArticle

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DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. / Graeber, Kai; Linkies, Ada; Steinbrecher, Tina; Mummenhoff, Klaus; Tarkowska, Danuse; Tureckova, Veronika; Ignatz, Michael; Sperber, Katja; Voegele, Antje; de Jong, Hans; Urbanova, Terezie; Strnad, Miroslav; Leubner-Metzger, Gerhard.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 34, 26.08.2014, p. E3571-E3580.

Research output: Contribution to journalArticle

Harvard

Graeber, K, Linkies, A, Steinbrecher, T, Mummenhoff, K, Tarkowska, D, Tureckova, V, Ignatz, M, Sperber, K, Voegele, A, de Jong, H, Urbanova, T, Strnad, M & Leubner-Metzger, G 2014, 'DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 34, pp. E3571-E3580. https://doi.org/10.1073/pnas.1403851111

APA

Graeber, K., Linkies, A., Steinbrecher, T., Mummenhoff, K., Tarkowska, D., Tureckova, V., Ignatz, M., Sperber, K., Voegele, A., de Jong, H., Urbanova, T., Strnad, M., & Leubner-Metzger, G. (2014). DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. Proceedings of the National Academy of Sciences of the United States of America, 111(34), E3571-E3580. https://doi.org/10.1073/pnas.1403851111

Vancouver

Graeber K, Linkies A, Steinbrecher T, Mummenhoff K, Tarkowska D, Tureckova V et al. DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. Proceedings of the National Academy of Sciences of the United States of America. 2014 Aug 26;111(34):E3571-E3580. https://doi.org/10.1073/pnas.1403851111

Author

Graeber, Kai ; Linkies, Ada ; Steinbrecher, Tina ; Mummenhoff, Klaus ; Tarkowska, Danuse ; Tureckova, Veronika ; Ignatz, Michael ; Sperber, Katja ; Voegele, Antje ; de Jong, Hans ; Urbanova, Terezie ; Strnad, Miroslav ; Leubner-Metzger, Gerhard. / DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 34. pp. E3571-E3580.

BibTeX

@article{d8d7b3dd9dc041e3b1bd62d2602d57e8,
title = "DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination",
abstract = "Seed germination is an important life-cycle transition because it determines subsequent plant survival and reproductive success. To detect optimal spatiotemporal conditions for germination, seeds act as sophisticated environmental sensors integrating information such as ambient temperature. Here we show that the DELAY OF GERMINATION 1 (DOG1) gene, known for providing dormancy adaptation to distinct environments, determines the optimal temperature for seed germination. By reciprocal gene-swapping experiments between Brassicaceae species we show that the DOG1-mediated dormancy mechanism is conserved. Biomechanical analyses show that this mechanism regulates the material properties of the endosperm, a seed tissue layer acting as germination barrier to control coat dormancy. We found that DOG1 inhibits the expression of gibberellin (GA)-regulated genes encoding cell-wall remodeling proteins in a temperature-dependent manner. Furthermore we demonstrate that DOG1 causes temperature-dependent alterations in the seed GA metabolism. These alterations in hormone metabolism are brought about by the temperature-dependent differential expression of genes encoding key enzymes of the GA biosynthetic pathway. These effects of DOG1 lead to a temperature-dependent control of endosperm weakening and determine the optimal temperature for germination. The conserved DOG1-mediated coat-dormancy mechanism provides a highly adaptable temperature-sensing mechanism to control the timing of germination.",
author = "Kai Graeber and Ada Linkies and Tina Steinbrecher and Klaus Mummenhoff and Danuse Tarkowska and Veronika Tureckova and Michael Ignatz and Katja Sperber and Antje Voegele and {de Jong}, Hans and Terezie Urbanova and Miroslav Strnad and Gerhard Leubner-Metzger",
year = "2014",
month = aug
day = "26",
doi = "10.1073/pnas.1403851111",
language = "English",
volume = "111",
pages = "E3571--E3580",
journal = " Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "34",

}

RIS

TY - JOUR

T1 - DELAY OF GERMINATION 1 mediates a conserved coat-dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination

AU - Graeber, Kai

AU - Linkies, Ada

AU - Steinbrecher, Tina

AU - Mummenhoff, Klaus

AU - Tarkowska, Danuse

AU - Tureckova, Veronika

AU - Ignatz, Michael

AU - Sperber, Katja

AU - Voegele, Antje

AU - de Jong, Hans

AU - Urbanova, Terezie

AU - Strnad, Miroslav

AU - Leubner-Metzger, Gerhard

PY - 2014/8/26

Y1 - 2014/8/26

N2 - Seed germination is an important life-cycle transition because it determines subsequent plant survival and reproductive success. To detect optimal spatiotemporal conditions for germination, seeds act as sophisticated environmental sensors integrating information such as ambient temperature. Here we show that the DELAY OF GERMINATION 1 (DOG1) gene, known for providing dormancy adaptation to distinct environments, determines the optimal temperature for seed germination. By reciprocal gene-swapping experiments between Brassicaceae species we show that the DOG1-mediated dormancy mechanism is conserved. Biomechanical analyses show that this mechanism regulates the material properties of the endosperm, a seed tissue layer acting as germination barrier to control coat dormancy. We found that DOG1 inhibits the expression of gibberellin (GA)-regulated genes encoding cell-wall remodeling proteins in a temperature-dependent manner. Furthermore we demonstrate that DOG1 causes temperature-dependent alterations in the seed GA metabolism. These alterations in hormone metabolism are brought about by the temperature-dependent differential expression of genes encoding key enzymes of the GA biosynthetic pathway. These effects of DOG1 lead to a temperature-dependent control of endosperm weakening and determine the optimal temperature for germination. The conserved DOG1-mediated coat-dormancy mechanism provides a highly adaptable temperature-sensing mechanism to control the timing of germination.

AB - Seed germination is an important life-cycle transition because it determines subsequent plant survival and reproductive success. To detect optimal spatiotemporal conditions for germination, seeds act as sophisticated environmental sensors integrating information such as ambient temperature. Here we show that the DELAY OF GERMINATION 1 (DOG1) gene, known for providing dormancy adaptation to distinct environments, determines the optimal temperature for seed germination. By reciprocal gene-swapping experiments between Brassicaceae species we show that the DOG1-mediated dormancy mechanism is conserved. Biomechanical analyses show that this mechanism regulates the material properties of the endosperm, a seed tissue layer acting as germination barrier to control coat dormancy. We found that DOG1 inhibits the expression of gibberellin (GA)-regulated genes encoding cell-wall remodeling proteins in a temperature-dependent manner. Furthermore we demonstrate that DOG1 causes temperature-dependent alterations in the seed GA metabolism. These alterations in hormone metabolism are brought about by the temperature-dependent differential expression of genes encoding key enzymes of the GA biosynthetic pathway. These effects of DOG1 lead to a temperature-dependent control of endosperm weakening and determine the optimal temperature for germination. The conserved DOG1-mediated coat-dormancy mechanism provides a highly adaptable temperature-sensing mechanism to control the timing of germination.

U2 - 10.1073/pnas.1403851111

DO - 10.1073/pnas.1403851111

M3 - Article

VL - 111

SP - E3571-E3580

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 34

ER -