DELAY OF GERMINATION 1 mediates a conserved coat dormancy mechanism for the temperature- and gibberellin-dependent control of seed germination. / Graeber, Kai; Linkies, Ada; Steinbrecher, Tina; Mummenhoff, Klaus; Tarkowska, Danuse; Tureckova, Veronika; Ignatz, Michael; Sperber, Katja; Voegele, Antje; de Jong, Hans; Urbanova, Terezie; Strnad, Miroslav; Leubner-Metzger, Gerhard.

In: Proceedings of the National Academy of Sciences of the United States of America, 11.08.2014, p. E3571-E3580.

Research output: Contribution to journalArticle

Published

Documents

Links

Abstract

Seed germination is an important life-cycle transition because it
determines subsequent plant survival and reproductive success. To
detect optimal spatiotemporal conditions for germination, seeds act
as sophisticated environmental sensors integrating information such
as ambient temperature. Here we show that the DELAY OF GERMINATION
1 (DOG1) gene, known for providing dormancy adaptation
to distinct environments, determines the optimal temperature for
seed germination. By reciprocal gene-swapping experiments between
Brassicaceae species we show that the DOG1-mediated dormancy
mechanism is conserved. Biomechanical analyses showthat thismechanism
regulates the material properties of the endosperm, a seed
tissue layer acting as germination barrier to control coat dormancy.
We found that DOG1 inhibits the expression of gibberellin (GA)-regulated
genes encoding cell-wall remodeling proteins in a temperaturedependent
manner. Furthermore we demonstrate that DOG1 causes
temperature-dependent alterations in the seed GA metabolism. These
alterations in hormone metabolism are brought about by the temperature-
dependent differential expression of genes encoding key
enzymes of the GA biosynthetic pathway. These effects of DOG1 lead
to a temperature-dependent control of endosperm weakening and
determine the optimal temperature for germination. The conserved
DOG1-mediated coat-dormancymechanismprovides a highly adaptable
temperature-sensing mechanism to control the timing of germination.
Original languageEnglish
Pages (from-to)E3571-E3580
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Early online date11 Aug 2014
DOIs
StatePublished - 11 Aug 2014
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

ID: 22623140