Chloroplast Biogenesis-Associated Nuclear Genes : Control by Plastid Signals Evolved Prior to Their Regulation as Part of Photomorphogenesis. / Hills, Alison C; Khan, Safina; Lopez Juez, Enrique.

In: Frontiers in Plant Science, Vol. 6, 1078, 10.12.2015, p. 1-13.

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Chloroplast Biogenesis-Associated Nuclear Genes : Control by Plastid Signals Evolved Prior to Their Regulation as Part of Photomorphogenesis. / Hills, Alison C; Khan, Safina; Lopez Juez, Enrique.

In: Frontiers in Plant Science, Vol. 6, 1078, 10.12.2015, p. 1-13.

Research output: Contribution to journalArticle

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@article{597a78465e254a4099680ec23c2e7efb,
title = "Chloroplast Biogenesis-Associated Nuclear Genes: Control by Plastid Signals Evolved Prior to Their Regulation as Part of Photomorphogenesis",
abstract = "The assembly of photosynthetically-competent chloroplasts occurs in angiosperm seedlings when first exposed to light, and is due to the control by light of photosynthesis-associated nuclear genes (PhANGs), also dependent upon plastid-to-nucleus “biogenic” communication signals. The relationship between light- and plastid signal-regulation of PhANGs is close but poorly understood. In contrast, many conifers green in the dark and the promoter of a pine PhANG, Lhcb, is active in the dark in tobacco. Here we show that the activity of this promoter in tobacco is sensitive to plastid photobleaching, or to the inhibition of plastid translation in the light or the dark, and the same interventions reduce expression of the native gene in pine seedlings, demonstrating classic plastid biogenic signalling in gymnosperms. Furthermore, Arabidopsis mutations causing defective plastid biogenesis suppress the effect in darkness of mutations in COP1 and DET1, repressors of photomorphogenesis, for the expression of several PhANGs but not a photosynthesis-unrelated, light-regulated gene. GLK transcriptional regulators mediate the response of LHCB but not of other tested PhANGs. We propose the ability to suppress PhANG response to positive plastid biogenic signals in the dark may have contributed to the evolution of light controlled chloroplast biogenesis.",
keywords = "Chloroplast development, photomorphogenesis, plastid signals, LHCB, DET1, COP1 Arabidopsis, GLK, gymnosperm photomorphogenesis",
author = "Hills, {Alison C} and Safina Khan and {Lopez Juez}, Enrique",
year = "2015",
month = dec
day = "10",
doi = "10.3389/fpls.2015.01078",
language = "English",
volume = "6",
pages = "1--13",
journal = "Frontiers in Plant Science",
issn = "1664-462X",
publisher = "Frontiers Media S.A.",

}

RIS

TY - JOUR

T1 - Chloroplast Biogenesis-Associated Nuclear Genes

T2 - Control by Plastid Signals Evolved Prior to Their Regulation as Part of Photomorphogenesis

AU - Hills, Alison C

AU - Khan, Safina

AU - Lopez Juez, Enrique

PY - 2015/12/10

Y1 - 2015/12/10

N2 - The assembly of photosynthetically-competent chloroplasts occurs in angiosperm seedlings when first exposed to light, and is due to the control by light of photosynthesis-associated nuclear genes (PhANGs), also dependent upon plastid-to-nucleus “biogenic” communication signals. The relationship between light- and plastid signal-regulation of PhANGs is close but poorly understood. In contrast, many conifers green in the dark and the promoter of a pine PhANG, Lhcb, is active in the dark in tobacco. Here we show that the activity of this promoter in tobacco is sensitive to plastid photobleaching, or to the inhibition of plastid translation in the light or the dark, and the same interventions reduce expression of the native gene in pine seedlings, demonstrating classic plastid biogenic signalling in gymnosperms. Furthermore, Arabidopsis mutations causing defective plastid biogenesis suppress the effect in darkness of mutations in COP1 and DET1, repressors of photomorphogenesis, for the expression of several PhANGs but not a photosynthesis-unrelated, light-regulated gene. GLK transcriptional regulators mediate the response of LHCB but not of other tested PhANGs. We propose the ability to suppress PhANG response to positive plastid biogenic signals in the dark may have contributed to the evolution of light controlled chloroplast biogenesis.

AB - The assembly of photosynthetically-competent chloroplasts occurs in angiosperm seedlings when first exposed to light, and is due to the control by light of photosynthesis-associated nuclear genes (PhANGs), also dependent upon plastid-to-nucleus “biogenic” communication signals. The relationship between light- and plastid signal-regulation of PhANGs is close but poorly understood. In contrast, many conifers green in the dark and the promoter of a pine PhANG, Lhcb, is active in the dark in tobacco. Here we show that the activity of this promoter in tobacco is sensitive to plastid photobleaching, or to the inhibition of plastid translation in the light or the dark, and the same interventions reduce expression of the native gene in pine seedlings, demonstrating classic plastid biogenic signalling in gymnosperms. Furthermore, Arabidopsis mutations causing defective plastid biogenesis suppress the effect in darkness of mutations in COP1 and DET1, repressors of photomorphogenesis, for the expression of several PhANGs but not a photosynthesis-unrelated, light-regulated gene. GLK transcriptional regulators mediate the response of LHCB but not of other tested PhANGs. We propose the ability to suppress PhANG response to positive plastid biogenic signals in the dark may have contributed to the evolution of light controlled chloroplast biogenesis.

KW - Chloroplast development

KW - photomorphogenesis

KW - plastid signals

KW - LHCB

KW - DET1

KW - COP1 Arabidopsis

KW - GLK

KW - gymnosperm photomorphogenesis

U2 - 10.3389/fpls.2015.01078

DO - 10.3389/fpls.2015.01078

M3 - Article

VL - 6

SP - 1

EP - 13

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

SN - 1664-462X

M1 - 1078

ER -