Screening carotenoids of elite accessions of yam (Dioscorea spp.) used in the global yam breeding program has been conducted to quantitatively determine the carotenoid composition of the crop. Comparisons to previous data reporting caerotenoid levels in yam has been made in order to deduce greater perspectives across multiple studies. as previous reports are sparse and conflicting. Characterisation of complex species and accession -specific profiles have shown a rich base of diversity that can inform breeding strategies. Key findings include; (i) the identification of accessions rich in β-carotene which can aid prov-Vitamin A biofortification, (ii), Ddata contrasting to the commonly held belief that yellow Guinea yam (D. cayennensis) has higher β-carotene content than that of white Guinea yam (D. rotundata), and (iii) the tentative identification of C25-epoxy-apocarotenoid persicaxanthin with potential implications for tuber dormancy.