Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.). / Whigham, Ehren; Qi, Shan; Mistry, Divya; Surana, Priyanka; Xu, Ruo; Fuerst, Gregory; Pliego, Clara; Bindschedler, Laurence; Spanu, Pietro D.; Dickerson, Julie A; Innes, Roger W; Nettleton, Dan; Bogdanove, Adam J; Wise, Roger P.

In: Molecular plant-Microbe interactions, Vol. 28, No. 9, 11.09.2015, p. 968-983.

Research output: Contribution to journalArticlepeer-review

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Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.). / Whigham, Ehren; Qi, Shan; Mistry, Divya; Surana, Priyanka; Xu, Ruo; Fuerst, Gregory; Pliego, Clara; Bindschedler, Laurence; Spanu, Pietro D.; Dickerson, Julie A; Innes, Roger W; Nettleton, Dan; Bogdanove, Adam J; Wise, Roger P.

In: Molecular plant-Microbe interactions, Vol. 28, No. 9, 11.09.2015, p. 968-983.

Research output: Contribution to journalArticlepeer-review

Harvard

Whigham, E, Qi, S, Mistry, D, Surana, P, Xu, R, Fuerst, G, Pliego, C, Bindschedler, L, Spanu, PD, Dickerson, JA, Innes, RW, Nettleton, D, Bogdanove, AJ & Wise, RP 2015, 'Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.)', Molecular plant-Microbe interactions, vol. 28, no. 9, pp. 968-983. https://doi.org/10.1094/MPMI-02-15-0027-FI

APA

Whigham, E., Qi, S., Mistry, D., Surana, P., Xu, R., Fuerst, G., Pliego, C., Bindschedler, L., Spanu, P. D., Dickerson, J. A., Innes, R. W., Nettleton, D., Bogdanove, A. J., & Wise, R. P. (2015). Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.). Molecular plant-Microbe interactions, 28(9), 968-983. https://doi.org/10.1094/MPMI-02-15-0027-FI

Vancouver

Author

Whigham, Ehren ; Qi, Shan ; Mistry, Divya ; Surana, Priyanka ; Xu, Ruo ; Fuerst, Gregory ; Pliego, Clara ; Bindschedler, Laurence ; Spanu, Pietro D. ; Dickerson, Julie A ; Innes, Roger W ; Nettleton, Dan ; Bogdanove, Adam J ; Wise, Roger P. / Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.). In: Molecular plant-Microbe interactions. 2015 ; Vol. 28, No. 9. pp. 968-983.

BibTeX

@article{514a8805232b46709a3899c768a5dd47,
title = "Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.)",
abstract = "The interaction of barley, Hordeum vulgare L., with the powdery mildew fungus Blumeria graminis f. sp. hordei is a well-developed model to investigate resistance and susceptibility to obligate biotrophic pathogens. The 130-Mb Blumeria genome encodes approximately 540 predicted effectors that are hypothesized to suppress or induce host processes to promote colonization. Blumeria effector candidate (BEC)1019, a single-copy gene encoding a putative, secreted metalloprotease, is expressed in haustorial feeding structures, and host-induced gene silencing of BEC1019 restricts haustorial development in compatible interactions. Here, we show that Barley stripe mosaic virus–induced gene silencing of BEC1019 significantly reduces fungal colonization of barley epidermal cells, demonstrating that BEC1019 plays a central role in virulence. In addition, delivery of BEC1019 to the host cytoplasm via Xanthomonas type III secretion suppresses cultivar nonspecific hypersensitive reaction (HR) induced by Xanthomonas oryzae pv. oryzicola, as well as cultivar-specific HR induced by AvrPphB from Pseudomonas syringae pv. phaseolicola. BEC1019 homologs are present in 96 of 241 sequenced fungal genomes, including plant pathogens, human pathogens, and free-living nonpathogens. Comparative analysis revealed variation at several amino acid positions that correlate with fungal lifestyle and several highly conserved, noncorrelated motifs. Site-directed mutagenesis of one of these, ETVIC, compromises the HR-suppressing activity of BEC1019. We postulate that BEC1019 represents an ancient, broadly important fungal protein family, members of which have evolved to function as effectors in plant and animal hosts.",
author = "Ehren Whigham and Shan Qi and Divya Mistry and Priyanka Surana and Ruo Xu and Gregory Fuerst and Clara Pliego and Laurence Bindschedler and Spanu, {Pietro D.} and Dickerson, {Julie A} and Innes, {Roger W} and Dan Nettleton and Bogdanove, {Adam J} and Wise, {Roger P}",
year = "2015",
month = sep,
day = "11",
doi = "10.1094/MPMI-02-15-0027-FI",
language = "English",
volume = "28",
pages = "968--983",
journal = "Molecular plant-Microbe interactions",
issn = "0894-0282",
publisher = "American Phytopathological Society",
number = "9",

}

RIS

TY - JOUR

T1 - Broadly Conserved Fungal Effector BEC1019 Suppresses Host Cell Death and Enhances Pathogen Virulence in Powdery Mildew of Barley (Hordeum vulgare L.)

AU - Whigham, Ehren

AU - Qi, Shan

AU - Mistry, Divya

AU - Surana, Priyanka

AU - Xu, Ruo

AU - Fuerst, Gregory

AU - Pliego, Clara

AU - Bindschedler, Laurence

AU - Spanu, Pietro D.

AU - Dickerson, Julie A

AU - Innes, Roger W

AU - Nettleton, Dan

AU - Bogdanove, Adam J

AU - Wise, Roger P

PY - 2015/9/11

Y1 - 2015/9/11

N2 - The interaction of barley, Hordeum vulgare L., with the powdery mildew fungus Blumeria graminis f. sp. hordei is a well-developed model to investigate resistance and susceptibility to obligate biotrophic pathogens. The 130-Mb Blumeria genome encodes approximately 540 predicted effectors that are hypothesized to suppress or induce host processes to promote colonization. Blumeria effector candidate (BEC)1019, a single-copy gene encoding a putative, secreted metalloprotease, is expressed in haustorial feeding structures, and host-induced gene silencing of BEC1019 restricts haustorial development in compatible interactions. Here, we show that Barley stripe mosaic virus–induced gene silencing of BEC1019 significantly reduces fungal colonization of barley epidermal cells, demonstrating that BEC1019 plays a central role in virulence. In addition, delivery of BEC1019 to the host cytoplasm via Xanthomonas type III secretion suppresses cultivar nonspecific hypersensitive reaction (HR) induced by Xanthomonas oryzae pv. oryzicola, as well as cultivar-specific HR induced by AvrPphB from Pseudomonas syringae pv. phaseolicola. BEC1019 homologs are present in 96 of 241 sequenced fungal genomes, including plant pathogens, human pathogens, and free-living nonpathogens. Comparative analysis revealed variation at several amino acid positions that correlate with fungal lifestyle and several highly conserved, noncorrelated motifs. Site-directed mutagenesis of one of these, ETVIC, compromises the HR-suppressing activity of BEC1019. We postulate that BEC1019 represents an ancient, broadly important fungal protein family, members of which have evolved to function as effectors in plant and animal hosts.

AB - The interaction of barley, Hordeum vulgare L., with the powdery mildew fungus Blumeria graminis f. sp. hordei is a well-developed model to investigate resistance and susceptibility to obligate biotrophic pathogens. The 130-Mb Blumeria genome encodes approximately 540 predicted effectors that are hypothesized to suppress or induce host processes to promote colonization. Blumeria effector candidate (BEC)1019, a single-copy gene encoding a putative, secreted metalloprotease, is expressed in haustorial feeding structures, and host-induced gene silencing of BEC1019 restricts haustorial development in compatible interactions. Here, we show that Barley stripe mosaic virus–induced gene silencing of BEC1019 significantly reduces fungal colonization of barley epidermal cells, demonstrating that BEC1019 plays a central role in virulence. In addition, delivery of BEC1019 to the host cytoplasm via Xanthomonas type III secretion suppresses cultivar nonspecific hypersensitive reaction (HR) induced by Xanthomonas oryzae pv. oryzicola, as well as cultivar-specific HR induced by AvrPphB from Pseudomonas syringae pv. phaseolicola. BEC1019 homologs are present in 96 of 241 sequenced fungal genomes, including plant pathogens, human pathogens, and free-living nonpathogens. Comparative analysis revealed variation at several amino acid positions that correlate with fungal lifestyle and several highly conserved, noncorrelated motifs. Site-directed mutagenesis of one of these, ETVIC, compromises the HR-suppressing activity of BEC1019. We postulate that BEC1019 represents an ancient, broadly important fungal protein family, members of which have evolved to function as effectors in plant and animal hosts.

U2 - 10.1094/MPMI-02-15-0027-FI

DO - 10.1094/MPMI-02-15-0027-FI

M3 - Article

VL - 28

SP - 968

EP - 983

JO - Molecular plant-Microbe interactions

JF - Molecular plant-Microbe interactions

SN - 0894-0282

IS - 9

ER -