Attenuating the DNA damage response to double-strand breaks restores function in models of CNS neurodegeneration. / Tuxworth , Richard ; Taylor, Matthew ; Anduaga, Ane Martin ; Hussien-Ali, Al; Chatzimatthaiou, Sotiroula ; Longland, Joanne ; Thompson, Adam; Almutiri, Sharif ; Alifragis, Pavlos; Kyriacou, Charalambos ; Kysela, Boris ; Ahmed, Zubair .

In: Brain Communications, Vol. 1, No. 1, fcz005, 02.07.2019, p. 1-21.

Research output: Contribution to journalArticle

Published
  • Richard Tuxworth
  • Matthew Taylor
  • Ane Martin Anduaga
  • Al Hussien-Ali
  • Sotiroula Chatzimatthaiou
  • Joanne Longland
  • Adam Thompson
  • Sharif Almutiri
  • Pavlos Alifragis
  • Charalambos Kyriacou
  • Boris Kysela
  • Zubair Ahmed

Abstract

DNA double-strand breaks are a feature of many acute and long-term neurological disorders, including neurodegeneration, following neurotrauma and after stroke. Persistent activation of the DNA damage response in response to double-strand breaks contributes to neural dysfunction and pathology as it can force post-mitotic neurons to re-enter the cell cycle leading to senescence or apoptosis. Mature, non-dividing neurons may tolerate low levels of DNA damage, in which case muting the DNA damage response might be neuroprotective. Here, we show that attenuating the DNA damage response by targeting the meiotic recombination 11, Rad50, Nijmegen breakage syndrome 1 complex, which is involved in double-strand break recognition, is neuroprotective in three neurodegeneration models in Drosophila and prevents Aβ1-42-induced loss of synapses in embryonic hippocampal neurons. Attenuating the DNA damage response after optic nerve injury is also neuroprotective to retinal ganglion cells and promotes dramatic regeneration of their neurites both in vitro and in vivo. Dorsal root ganglion neurons similarly regenerate when the DNA damage response is targeted in vitro and in vivo and this strategy also induces significant restoration of lost function after spinal cord injury. We conclude that muting the DNA damage response in the nervous system is neuroprotective in multiple neurological disorders. Our results point to new therapies to maintain or repair the nervous system.
Original languageEnglish
Article numberfcz005
Pages (from-to)1-21
Number of pages21
JournalBrain Communications
Volume1
Issue number1
DOIs
Publication statusPublished - 2 Jul 2019
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

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