Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. / Horvath, Beatrix; Kourova, Hana ; Nagy, Szilvia ; Nemeth, Edit; Magyar, Zoltan ; Papdi, Csaba; Ahmad, Zaki; Sanchez-Perez, Gabino F. ; Perilli, Serena ; Blilou, Ikram; Pettkó-Szandtner, Aladár ; Darula, Zsuzsanna ; Meszaros, Tamas ; Binarova, Pavla ; Bogre, Laszlo; Scheres, Ben.

In: EMBO Journal , Vol. 36, No. 9, 02.05.2017, p. 1261-1278.

Research output: Contribution to journalArticle

Published

Standard

Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. / Horvath, Beatrix; Kourova, Hana ; Nagy, Szilvia ; Nemeth, Edit; Magyar, Zoltan ; Papdi, Csaba; Ahmad, Zaki; Sanchez-Perez, Gabino F. ; Perilli, Serena ; Blilou, Ikram; Pettkó-Szandtner, Aladár ; Darula, Zsuzsanna ; Meszaros, Tamas ; Binarova, Pavla ; Bogre, Laszlo; Scheres, Ben.

In: EMBO Journal , Vol. 36, No. 9, 02.05.2017, p. 1261-1278.

Research output: Contribution to journalArticle

Harvard

Horvath, B, Kourova, H, Nagy, S, Nemeth, E, Magyar, Z, Papdi, C, Ahmad, Z, Sanchez-Perez, GF, Perilli, S, Blilou, I, Pettkó-Szandtner, A, Darula, Z, Meszaros, T, Binarova, P, Bogre, L & Scheres, B 2017, 'Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control', EMBO Journal , vol. 36, no. 9, pp. 1261-1278. https://doi.org/10.15252/embj.201694561

APA

Horvath, B., Kourova, H., Nagy, S., Nemeth, E., Magyar, Z., Papdi, C., Ahmad, Z., Sanchez-Perez, G. F., Perilli, S., Blilou, I., Pettkó-Szandtner, A., Darula, Z., Meszaros, T., Binarova, P., Bogre, L., & Scheres, B. (2017). Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. EMBO Journal , 36(9), 1261-1278. https://doi.org/10.15252/embj.201694561

Vancouver

Author

Horvath, Beatrix ; Kourova, Hana ; Nagy, Szilvia ; Nemeth, Edit ; Magyar, Zoltan ; Papdi, Csaba ; Ahmad, Zaki ; Sanchez-Perez, Gabino F. ; Perilli, Serena ; Blilou, Ikram ; Pettkó-Szandtner, Aladár ; Darula, Zsuzsanna ; Meszaros, Tamas ; Binarova, Pavla ; Bogre, Laszlo ; Scheres, Ben. / Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. In: EMBO Journal . 2017 ; Vol. 36, No. 9. pp. 1261-1278.

BibTeX

@article{4193112f7d754ffe8b051cd6b1ba73b7,
title = "Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control",
abstract = "The rapidly proliferating cells in plant meristems must be protected from genome damage. Here we show that the regulatory role of the Arabidopsis RETINOBLASTOMA RELATED (RBR) in cell proliferation can be separated from a novel function in safeguarding genome integrity. Upon DNA damage, RBR and its binding partner E2FA are recruited to heterochromatic γH2AX- labelled DNA damage foci in an ATM and ATRdependent manner. These γH2AX labelled DNA lesions are more dispersedly occupied by the conserved repair protein, AtBRCA1, which can also co-localise with RBR foci. RBR and AtBRCA1 physically interact in vitro and in planta. Genetic interaction between the RBR-silenced amiRBR and Atbrca1 mutants suggests that RBR and AtBRCA1 may function together in maintaining genome integrity. Together with E2FA, RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG1, the plant functional analogue of p53. Thus, plant homologs and analogues of major mammalian tumour suppressor proteins form a regulatory network that coordinates cell proliferation with cell and genome integrity.",
keywords = "Arabidopsis, BRCA1, DNA damage response, E2FA, RETINOBLASTOMA-RELATED",
author = "Beatrix Horvath and Hana Kourova and Szilvia Nagy and Edit Nemeth and Zoltan Magyar and Csaba Papdi and Zaki Ahmad and Sanchez-Perez, {Gabino F.} and Serena Perilli and Ikram Blilou and Alad{\'a}r Pettk{\'o}-Szandtner and Zsuzsanna Darula and Tamas Meszaros and Pavla Binarova and Laszlo Bogre and Ben Scheres",
year = "2017",
month = may,
day = "2",
doi = "10.15252/embj.201694561",
language = "English",
volume = "36",
pages = "1261--1278",
journal = "EMBO Journal ",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "9",

}

RIS

TY - JOUR

T1 - Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control

AU - Horvath, Beatrix

AU - Kourova, Hana

AU - Nagy, Szilvia

AU - Nemeth, Edit

AU - Magyar, Zoltan

AU - Papdi, Csaba

AU - Ahmad, Zaki

AU - Sanchez-Perez, Gabino F.

AU - Perilli, Serena

AU - Blilou, Ikram

AU - Pettkó-Szandtner, Aladár

AU - Darula, Zsuzsanna

AU - Meszaros, Tamas

AU - Binarova, Pavla

AU - Bogre, Laszlo

AU - Scheres, Ben

PY - 2017/5/2

Y1 - 2017/5/2

N2 - The rapidly proliferating cells in plant meristems must be protected from genome damage. Here we show that the regulatory role of the Arabidopsis RETINOBLASTOMA RELATED (RBR) in cell proliferation can be separated from a novel function in safeguarding genome integrity. Upon DNA damage, RBR and its binding partner E2FA are recruited to heterochromatic γH2AX- labelled DNA damage foci in an ATM and ATRdependent manner. These γH2AX labelled DNA lesions are more dispersedly occupied by the conserved repair protein, AtBRCA1, which can also co-localise with RBR foci. RBR and AtBRCA1 physically interact in vitro and in planta. Genetic interaction between the RBR-silenced amiRBR and Atbrca1 mutants suggests that RBR and AtBRCA1 may function together in maintaining genome integrity. Together with E2FA, RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG1, the plant functional analogue of p53. Thus, plant homologs and analogues of major mammalian tumour suppressor proteins form a regulatory network that coordinates cell proliferation with cell and genome integrity.

AB - The rapidly proliferating cells in plant meristems must be protected from genome damage. Here we show that the regulatory role of the Arabidopsis RETINOBLASTOMA RELATED (RBR) in cell proliferation can be separated from a novel function in safeguarding genome integrity. Upon DNA damage, RBR and its binding partner E2FA are recruited to heterochromatic γH2AX- labelled DNA damage foci in an ATM and ATRdependent manner. These γH2AX labelled DNA lesions are more dispersedly occupied by the conserved repair protein, AtBRCA1, which can also co-localise with RBR foci. RBR and AtBRCA1 physically interact in vitro and in planta. Genetic interaction between the RBR-silenced amiRBR and Atbrca1 mutants suggests that RBR and AtBRCA1 may function together in maintaining genome integrity. Together with E2FA, RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG1, the plant functional analogue of p53. Thus, plant homologs and analogues of major mammalian tumour suppressor proteins form a regulatory network that coordinates cell proliferation with cell and genome integrity.

KW - Arabidopsis, BRCA1, DNA damage response, E2FA, RETINOBLASTOMA-RELATED

U2 - 10.15252/embj.201694561

DO - 10.15252/embj.201694561

M3 - Article

VL - 36

SP - 1261

EP - 1278

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 9

ER -