An intuitive graphical visualization technique for the interrogation of transcriptome data. / Bushati, Natascha; Smith, James; Briscoe, James; Watkins, Christopher.

In: Nucleic Acids Research, Vol. 39, No. 17, 2011, p. 7380-7389.

Research output: Contribution to journalArticle

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Abstract

The complexity of gene expression data sets generated from microarrays and high-throughput sequencing make their analysis challenging. One goal of these analyses is to define sets of co-regulated genes and identify patterns of gene expression. To date, however, there is a lack of easily implemented methods that allow an investigator to visualize and interact with the data in an intuitive and flexible manner. Here, we show that combining a nonlinear dimensionality reduction method, t-statistic Stochastic Neighbor Embedding, with a novel visualization technique provides a graphical mapping that allows the intuitive investigation of transcriptome data.
This approach performs better than commonly used methods, offering insight into underlying patterns of gene expression at both global and local scales and identifying clusters of similarly expressed genes. A freely available MATLAB-implemented graphical user interface to perform t-SNE and nearest neighbour plots on genomic data sets is available at www.nimr.mrc.ac.uk/research/james-briscoe/
visgenex
Original languageEnglish
Pages (from-to)7380-7389
JournalNucleic Acids Research
Volume39
Issue number17
Early online date19 Jun 2011
DOIs
Publication statusPublished - 2011
This open access research output is licenced under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License.

ID: 1854254