A transcriptomic, metabolomic and cellular approach to the physiological adaptation of tomato fruit to high temperature. / Almeida Barros da Silva, Juliana; Perez, Laura; Fraser, Paul.

In: Plant, Cell and Environment, 21.07.2020.

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@article{309a512cc01f44118f0fbc5cdc4e96ab,
title = "A transcriptomic, metabolomic and cellular approach to the physiological adaptation of tomato fruit to high temperature",
abstract = "High temperatures can negatively influence plant growth and development. Besides yield, the effects of heat stress on fruit quality traits remain poorly characterised. In tomato, insights into how fruits regulate cellular metabolism in response to heat stress could contribute to the development of heat-tolerant varieties, without detrimental effects on quality. In the present study, the changes occurring in wild type tomato fruits after exposure to transient heat stress have been elucidated at the transcriptome, cellular and metabolite level. An impact on fruit quality was evident as nutritional attributes changed in response to heat stress. Fruit carotenogenesis was affected, predominantly at the stage of phytoene formation, although altered desaturation/isomerisation arose during the transient exposure to high temperatures. Plastidial isoprenoid compounds showed subtle alterations in their distribution within chromoplast sub-compartments. Metabolite profiling suggests limited effects on primary/intermediary metabolism but lipid remodelling was evident. The heat-induced molecular signatures included the accumulation of sucrose and triacylglycerols, and a decrease in the degree of membrane lipid unsaturation, which influenced the volatile profile. Collectively, these data provide valuable insights into the underlying biochemical and molecular adaptation of fruit to heat stress and will impact on our ability to develop future climate resilient tomato varieties.",
keywords = "Heat stress; Tomato; Fruit quality; Transcriptomics, Metabolomics, Isoprenoids, Carotenoids, Plastoglobuli, Fruit ripening",
author = "{Almeida Barros da Silva}, Juliana and Laura Perez and Paul Fraser",
year = "2020",
month = jul,
day = "21",
doi = "10.1111/pce.13854",
language = "English",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley-Blackwell",

}

RIS

TY - JOUR

T1 - A transcriptomic, metabolomic and cellular approach to the physiological adaptation of tomato fruit to high temperature

AU - Almeida Barros da Silva, Juliana

AU - Perez, Laura

AU - Fraser, Paul

PY - 2020/7/21

Y1 - 2020/7/21

N2 - High temperatures can negatively influence plant growth and development. Besides yield, the effects of heat stress on fruit quality traits remain poorly characterised. In tomato, insights into how fruits regulate cellular metabolism in response to heat stress could contribute to the development of heat-tolerant varieties, without detrimental effects on quality. In the present study, the changes occurring in wild type tomato fruits after exposure to transient heat stress have been elucidated at the transcriptome, cellular and metabolite level. An impact on fruit quality was evident as nutritional attributes changed in response to heat stress. Fruit carotenogenesis was affected, predominantly at the stage of phytoene formation, although altered desaturation/isomerisation arose during the transient exposure to high temperatures. Plastidial isoprenoid compounds showed subtle alterations in their distribution within chromoplast sub-compartments. Metabolite profiling suggests limited effects on primary/intermediary metabolism but lipid remodelling was evident. The heat-induced molecular signatures included the accumulation of sucrose and triacylglycerols, and a decrease in the degree of membrane lipid unsaturation, which influenced the volatile profile. Collectively, these data provide valuable insights into the underlying biochemical and molecular adaptation of fruit to heat stress and will impact on our ability to develop future climate resilient tomato varieties.

AB - High temperatures can negatively influence plant growth and development. Besides yield, the effects of heat stress on fruit quality traits remain poorly characterised. In tomato, insights into how fruits regulate cellular metabolism in response to heat stress could contribute to the development of heat-tolerant varieties, without detrimental effects on quality. In the present study, the changes occurring in wild type tomato fruits after exposure to transient heat stress have been elucidated at the transcriptome, cellular and metabolite level. An impact on fruit quality was evident as nutritional attributes changed in response to heat stress. Fruit carotenogenesis was affected, predominantly at the stage of phytoene formation, although altered desaturation/isomerisation arose during the transient exposure to high temperatures. Plastidial isoprenoid compounds showed subtle alterations in their distribution within chromoplast sub-compartments. Metabolite profiling suggests limited effects on primary/intermediary metabolism but lipid remodelling was evident. The heat-induced molecular signatures included the accumulation of sucrose and triacylglycerols, and a decrease in the degree of membrane lipid unsaturation, which influenced the volatile profile. Collectively, these data provide valuable insights into the underlying biochemical and molecular adaptation of fruit to heat stress and will impact on our ability to develop future climate resilient tomato varieties.

KW - Heat stress; Tomato; Fruit quality; Transcriptomics, Metabolomics, Isoprenoids, Carotenoids, Plastoglobuli, Fruit ripening

U2 - 10.1111/pce.13854

DO - 10.1111/pce.13854

M3 - Article

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

ER -