TY - JOUR
T1 - Molecular mechanisms of Arabidopsis seed dormancy release by gas plasma activated water technology
AU - Grainge, Giles
AU - Nakabayashi, Kazumi
AU - Steinbrecher, Tina
AU - Kennedy, Sue
AU - Ren, Junchen
AU - Iza, Felipe
AU - Leubner-Metzger, Gerhard
PY - 2022/4/15
Y1 - 2022/4/15
N2 - Developing innovative agri-technologies is essential for the sustainable intensification of global food production. Seed dormancy is an adaptive trait which defines the environmental conditions in which the seed is able to germinate. Dormancy release requires sensing and integration of multiple environmental signals, a complex process which may be mimicked by seed treatment technologies. Here, we reveal the molecular mechanisms by which non-thermal (cold) atmospheric gas plasma activated water (GPAW) releases the physiological seed dormancy of Arabidopsis thaliana. GPAW triggered dormancy release by synergistic interaction between plasma generated reactive chemical species (NO3-, H2O2, •NO, •OH) and multiple signalling pathways targeting gibberellin and abscisic acid (ABA) metabolism and the expression of downstream cell wall remodelling genes. Direct chemical action of GPAW on cell walls resulted in premature biomechanical endosperm weakening. The germination responses of dormancy signalling (nlp8, prt6, dog1) and ABA metabolism (cyp707a2) mutants varied with GPAW composition. GPAW removes seed dormancy blocks by triggering multiple molecular signalling pathways combined with direct chemical tissue weakening to permit seed germination. Gas plasma technologies therefore improve seed quality by mimicking permissive environments in which sensing and integration of multiple signals leads to dormancy release and germination.
AB - Developing innovative agri-technologies is essential for the sustainable intensification of global food production. Seed dormancy is an adaptive trait which defines the environmental conditions in which the seed is able to germinate. Dormancy release requires sensing and integration of multiple environmental signals, a complex process which may be mimicked by seed treatment technologies. Here, we reveal the molecular mechanisms by which non-thermal (cold) atmospheric gas plasma activated water (GPAW) releases the physiological seed dormancy of Arabidopsis thaliana. GPAW triggered dormancy release by synergistic interaction between plasma generated reactive chemical species (NO3-, H2O2, •NO, •OH) and multiple signalling pathways targeting gibberellin and abscisic acid (ABA) metabolism and the expression of downstream cell wall remodelling genes. Direct chemical action of GPAW on cell walls resulted in premature biomechanical endosperm weakening. The germination responses of dormancy signalling (nlp8, prt6, dog1) and ABA metabolism (cyp707a2) mutants varied with GPAW composition. GPAW removes seed dormancy blocks by triggering multiple molecular signalling pathways combined with direct chemical tissue weakening to permit seed germination. Gas plasma technologies therefore improve seed quality by mimicking permissive environments in which sensing and integration of multiple signals leads to dormancy release and germination.
U2 - 10.1093/jxb/erac150
DO - 10.1093/jxb/erac150
M3 - Article
SN - 0022-0957
JO - Journal of Experimental Botany
JF - Journal of Experimental Botany
ER -