Projects per year
Results. We performed high-throughput ChIP-Seq on the HDA6 mutant, axe1-5, and wild-type plants with or without methyl jasmonate (MeJA) treatment to assess changes in active H4ac and repressive H3K27me3 histone markers. Transcriptional regulation was investigated in parallel by microarray analysis in the same conditions. MeJA and HDA6-dependent histone modifications on genes for specialized metabolism; linolenic acid and phenylpropanoid pathways; and abiotic and biotic stress responses were identified. H4ac and H3K27me3 enrichment also differentially affects JAs and HDA6 mediated genome integrity and gene regulatory networks, substantiating the role of HDA6 interacting with specific families of transposable elements in planta and highlighting further specificity of action as well as novel targets of HDA6 in the context of JA signalling for abiotic and biotic stress responses.
Conclusions. The findings demonstrate functional overlap for MeJA and HDA6 in tuning plant developmental plasticity and response to stress at the histone modification level. MeJA and HDA6, nonetheless, maintain distinct activities on histone modifications to modulate genetic variability and to allow adaptation to environmental challenges.
- Arabidopsis, Abiotic, Biotic, Chromatin remodelling, Histone Deacetylase, Histone Modification, Jasmonate, Regulatory Network, Specialized metabolism, stress response
- 3 Finished
Analysis of the regulation of stress tolerance and growth to improve stress responses under water scarcity in crops
1/10/16 → 30/09/18
1/06/10 → 31/05/12
1/10/06 → 30/09/09