Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease

Ngoc Lu-Nguyen; Martin Broadstock; Rafael J. Yáñez-Muñoz

doi:10.1007/978-1-4939-3753-0_13

Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease

Ngoc Lu-Nguyen, Martin Broadstock, Rafael J. Yáñez-Muñoz

Research output: Contribution to journal › Article › peer-review

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Abstract

Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson’s disease, and (3) postmortem brain processing.

Original language	English
Pages (from-to)	175-184
Number of pages	10
Journal	Methods in Molecular Biology
Volume	1448
Early online date	18 Jun 2016
DOIs	https://doi.org/10.1007/978-1-4939-3753-0_13
Publication status	E-pub ahead of print - 18 Jun 2016

Access to Document

10.1007/978-1-4939-3753-0_13

Accepted ManuscriptAccepted author manuscript, 396 KB

Integration-deficient lentiviral vectors delivering neurotrophic factors for gene therapy of Parkinson’s disease
Lu-Nguyen, N. (Student), Broadstock, M. (Fellow) & Yáñez-Muñoz, R. J. (PI)
1/10/09 → 1/07/13
Project: Research

10th Annual Conference of The BSGCT (UK)
Lu-Nguyen, N. (Speaker)
2013
Activity: Talk or presentation › Oral presentation
Parkinson’s UK Research Conference (UK)
Lu-Nguyen, N. (Speaker)
2012
Activity: Talk or presentation › Oral presentation

College overseas research scholarship - RHUL
Lu-Nguyen, N. (Recipient), 2009
Prize: Prize (including medals and awards)

Cite this

@article{7e7aeea8e25045e3968ead5c92016d7a,

title = "Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson{\textquoteright}s Disease",

abstract = "Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson{\textquoteright}s disease, and (3) postmortem brain processing.",

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AU - Lu-Nguyen, Ngoc

AU - Broadstock, Martin

AU - Yáñez-Muñoz, Rafael J.

PY - 2016/6/18

Y1 - 2016/6/18

N2 - Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson’s disease, and (3) postmortem brain processing.

AB - Standard integration-proficient lentiviral vectors (IPLVs) are effective at much lower doses than other vector systems and have shown promise in several gene therapy approaches. Their main drawback is the potential risk of insertional mutagenesis. Novel biosafety-enhanced integration-deficient lentiviral vectors (IDLVs) offer a significant improvement and comparable transduction efficacy to their integrating counterparts in some central nervous system applications. We describe here methods for (1) production of IDLVs (and IPLVs), (2) IDLV/IPLV delivery into the striatum of a rat model of Parkinson’s disease, and (3) postmortem brain processing.

U2 - 10.1007/978-1-4939-3753-0_13

DO - 10.1007/978-1-4939-3753-0_13

M3 - Article

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SP - 175

EP - 184

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson’s Disease

Abstract

Access to Document

Projects

Integration-deficient lentiviral vectors delivering neurotrophic factors for gene therapy of Parkinson’s disease

Activities

10th Annual Conference of The BSGCT (UK)

Parkinson’s UK Research Conference (UK)

Prizes

College overseas research scholarship - RHUL

Cite this