Different outcomes of endurance and resistance exercise in skeletal muscles of Oculopharyngeal muscular dystrophy

Alexis Boulinguiez, Jamila Dhiab, Barbara Crisol, Laura Muraine, Ludovic Gaut, Rouxel Corentin, Justine Flaire, Hadidja-Rose Mouigni, Megane Lemaitre, Benoit Giroux, Lucie Audoux, Benjamin SaintPierre, Arnaud Ferry, Vincent Mouly, Gillian Butler-Browne, Elisa Negroni, Alberto Malerba, Capucine Trollet

Research output: Contribution to journalArticlepeer-review

Abstract

Background Exercise is widely considered to have beneficial impact on skeletal muscle aging. In addition, there are also several studies demonstrating a positive effect of exercise on muscular dystrophies. Oculopharyngeal muscular dystrophy (OPMD) is a late-onset autosomal dominant inherited neuromuscular disorder caused by mutations in the PAPBN1 gene. These mutations consist in short (1-8) and meiotically stable GCN trinucleotide repeat expansions in its coding region responsible for the formation of PAPBN1 intranuclear aggregates. This study aims to characterize the effects of two types of chronic exercise, resistance and endurance, on the OPMD skeletal muscle phenotype using a relevant murine model of OPMD.

Methods In this study, we tested two protocols of exercise. In the first, based on endurance exercise, FvB (wild-type) and A17 (OPMD) mice underwent a 6-week-long motorized treadmill protocol consisting in 3 sessions per week of running 20cm/s for 20 minutes. In the second protocol, based on resistance exercise generated by chronic mechanical overload (OVL), surgical removal of gastrocnemius and soleus muscles was performed, inducing hypertrophy of the plantaris muscle. In both types of exercise, muscles of A17 and FvB mice were compared to those of respective sedentary mice. For all the groups, force measurement, muscle histology and molecular analyses were conducted.

Results Following the endurance exercise protocol, we did not observe any major changes in the muscle physiological parameters, but an increase in the number of PABPN1 intranuclear aggregates and enhanced collagen deposition in the exercised A17 OPMD mice. In the resistance overload protocol, we also observed an increased collagen deposition in the A17 OPMD mice which was associated with larger muscle mass and fiber cross sectional area and increased absolute maximal force as well as a reduction in PABPN1 aggregate number.

Conclusions Running exercise and mechanical overload led to very different outcome in skeletal muscles of A17 mice. Both types of exercise enhanced collagen deposition but while the running protocol increased aggregates, the OVL reduced them. More importantly OVL reversed muscle atrophy and maximal force in the A17 mice. Our study performed in a relevant model gives an indication of the effect of different types of exercise on OPMD muscle which should be further evaluated in humans for future recommendations as a part of the lifestyle of individuals with OPMD.
Original languageEnglish
JournalbioRxiv
DOIs
Publication statusSubmitted - 14 Jan 2024

Keywords

  • Oculopharyngeal muscular dystrophy
  • OPMD
  • skeletal muscle
  • PAPBN1
  • inherited neuromuscular disorder

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