Abstract
Confocal laser scanning microscopy is an excellent tool for nondestructive imaging of arthropods and can provide detailed information on morphology including fine surface detail. A methodology is presented here for the visualization by confocal microscopy of arthropods, using brachyuran crab zoeal stages as examples and postprocessing techniques derived from micro-CT protocols to improve the final images. This protocol is divided into description of the preprocessing steps (cleaning, staining, digesting and mounting), confocal laser scanning microscopy and data visualization using open-source, freeware programs ImageJ and Drishti. The advantages of using ImageJ to standardize stack data and Drishti for surface rendering are discussed. The methodology has been comprehensively tested using data acquired from all four brands of confocal microscope (Leica, Nikon, Olympus and Zeiss).
Original language | English |
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Pages (from-to) | 307-323 |
Number of pages | 18 |
Journal | Journal of Microscopy |
Volume | 266 |
Issue number | 3 |
Early online date | 7 Mar 2017 |
DOIs | |
Publication status | Published - Jun 2017 |
Keywords
- Brachyuran crab larvae, confocal laser scanning microscopy, Drishti, ImageJ, visualization, 3D imaging.